Excitotoxic neuronal cell death during an oligodendrocyte-directed CD8+T cell attack in the CNS gray matter
- Nico Melzer†1Email author,
- Gordon Hicking†1,
- Stefan Bittner1,
- Nicole Bobak1,
- Kerstin Göbel1,
- Alexander M Herrmann1,
- Heinz Wiendl†1 and
- Sven G Meuth1, 2
© Melzer et al.; licensee BioMed Central Ltd. 2013
Received: 4 August 2013
Accepted: 24 September 2013
Published: 5 October 2013
Neural-antigen reactive cytotoxic CD8+ T cells contribute to neuronal dysfunction and degeneration in a variety of inflammatory CNS disorders. Facing excess numbers of target cells, CNS-invading CD8+ T cells cause neuronal cell death either via confined release of cytotoxic effector molecules towards neurons, or via spillover of cytotoxic effector molecules from 'leaky’ immunological synapses and non-confined release by CD8+ T cells themselves during serial and simultaneous killing of oligodendrocytes or astrocytes.
Wild-type and T cell receptor transgenic CD8+ T cells were stimulated in vitro, their activation status was assessed by flow cytometry, and supernatant glutamate levels were determined using an enzymatic assay. Expression regulation of molecules involved in vesicular glutamate release was examined by quantitative real-time PCR, and mechanisms of non-vesicular glutamate release were studied by pharmacological blocking experiments. The impact of CD8+ T cell-mediated glutamate liberation on neuronal viability was studied in acute brain slice preparations.
Following T cell receptor stimulation, CD8+ T cells acquire the molecular repertoire for vesicular glutamate release: (i) they upregulate expression of glutaminase required to generate glutamate via deamination of glutamine and (ii) they upregulate expression of vesicular proton-ATPase and vesicular glutamate transporters required for filling of vesicles with glutamate. Subsequently, CD8+ T cells release glutamate in a strictly stimulus-dependent manner. Upon repetitive T cell receptor stimulation, CD25high CD8+ T effector cells exhibit higher estimated single cell glutamate release rates than CD25low CD8+ T memory cells. Moreover, glutamate liberation by oligodendrocyte-reactive CD25high CD8+ T effector cells is capable of eliciting collateral excitotoxic cell death of neurons (despite glutamate re-uptake by glia cells and neurons) in intact CNS gray matter.
Glutamate release may represent a crucial effector pathway of neural-antigen reactive CD8+ T cells, contributing to excitotoxicity in CNS inflammation.
KeywordsGlutamate excitotoxicity Neuronal cell death CNS tissue damage CD8+ T cells
Cytotoxic CD8+ T cells directed against neuronal or glial antigens contribute to neuronal cell death in a variety of inflammatory CNS disorders [1–3]. They are capable of harming neuronal function and integrity either by directly targeting neurons and their neurites or by exerting their action via 'collateral’ mechanisms of neuronal damage that might follow destruction of oligodendrocytes (ODCs), myelin sheaths or astrocytes in the CNS gray and white matter .
Recently, we have shown that activated T cell receptor (TCR)-transgenic, ovalbumin (OVA)-reactive CD8+ T cells (OT-I cells ) are capable of initiating 'collateral’ neuronal apoptosis in the gray matter of otherwise non-inflamed acute brain slices from ODC-OVA mice selectively expressing OVA in ODCs under the control of a truncated myelin basic protein (MBP)-promotor . 'Collateral’ killing of neurons by OT-I cells was in part due to release of perforin (and granzymes) whereas Fas-ligand (FasL) - Fas-receptor (Fas) interactions were not relevant [7–9]. However, a certain part of the collateral neuronal cell death induced by CD8+ T cells remained unexplained [7, 10].
During culture of polyclonally activated CD3+ T cells, high levels of extracellular glutamate have been shown to accumulate, which are efficiently cleared if T cells are co-cultured with astrocytes . In turn, the T cell-derived glutamate elicits the release of thiols (cysteine, glutathione and cysteinyl-glycine) and lactate from astrocytes, which reduce neuronal apoptosis induced by oxidative stress . However, whether glutamate derived from T cells migrating through the intact CNS parenchyma and scanning for their cognate antigen may also exert excitotoxic neuronal cell death, is unclear at present.
Wild-type (WT), ODC-OVA  and OT-I  mice were kept at the animal facility of the University of Münster, Germany, and had access to food and water ad libitum. All experiments were conducted according to the German law of animal protection and were approved by local authorities. All transgenic mice were created on or back-crossed to a C57BL/6 genetic background for at least ten generations.
Isolation of splenocytes and stimulation of CD8+T cells
Spleens of WT or OT-I mice were removed and single cell suspensions were generated by mashing spleens through a 70 μm strainer and lysing red blood cells with ammonium-chloride-potassium (ACK) buffer. Splenocytes were cultured in glutamine-containing DMEM-based standard medium consisting of DMEM (BioWhittaker, Verviers, Belgium) supplemented with 5% FCS (PAA Laboratories, Pasching, Germany), 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco, Invitrogen, Darmstadt, Germany), 2 mM L-glutamine (PAA Laboratories, Pasching, Germany), 50 μM 2-mercaptoethanol (Gibco, Invitrogen, Darmstadt, Germany), 1% non-essential amino acids (BioWhittaker, Verviers, Belgium) and 25 μg/ml gentamicin (Gibco, Invitrogen, Darmstadt, Germany).
For in vitro T cell stimulation experiments, CD8+ T cells were isolated from WT splenocytes using the respective MACS® T cell isolation kit (Miltenyi, Bergisch Gladbach, Germany) according to the manufacturer’s instructions. This yielded a purity of about 90% of CD3+ CD8+ T cells (Additional file 1: Figure S1A, B). CD8+ T cells from WT mice were cultured for different periods of time at a density of 1 × 106/well on 24-well plates with anti-CD3/CD28 beads (Dynal Biotech, Oslo, Norway) at different bead-to-cell ratios in the absence or presence of certain glutamate release blockers (all from Sigma-Aldrich, München, Germany) or were left unstimulated. In a subset of experiments, CD8+ T cells from WT mice were stimulated in glutamine-free DMEM-based medium (BioWhittaker, Verviers, Belgium). After three days of stimulation, supernatants were removed and analyzed for IFNγ using a mouse-IFNγ-ELISA kit according to the manufacturer’s protocol (Duoset, R&D Systems, Wiesbaden, Germany).
For in vitro T cell stimulation experiments, splenocytes from OT-I mice were plated at a density of 1 × 107/well on 12-well plates and primed by incubation for five days with OVA-peptide257-264 (SIINFEKL; 1 nM) and IL-2 (500 U/ml). On day three, supernatants were removed, analyzed for IFNγ using a mouse-IFNγ-ELISA kit according to the manufacturer’s protocol (Duoset, R&D Systems, Wiesbaden, Germany) and substituted by fresh medium. On day four, again 500 U/ml of IL-2 were added to the medium. In a subset of experiments, OT-I T cells were repetitively stimulated by incubation with OVA-peptide257-264 (SIINFEKL; 1 nM) and IL-2 (500 U/ml) every three days for a total of fifteen days.
Before and after various time periods of in vitro culture, flow cytometry of stimulated WT CD8+ T cells and OT-I cells was performed using standard methods. For analysis of T cell subtype distribution, cells were stained for 20 minutes with allophycocyanin (APC)-labeled anti-mouse CD3, phycoerythrin (PE)-labeled anti-mouse CD8 and fluorescein isothiocyanate (FITC)-labeled anti-mouse CD4 (all BD Bioscience, Heidelberg, Germany). As isotype controls, cells were stained with APC-labeled anti-mouse IgG1, PE-labeled anti-mouse IgG1 or FITC-labeled anti-mouse IgG1 (all by BD Bioscience, Heidelberg, Germany). For analysis of T cell activation markers, cells were stained for 20 minutes with FITC-labeled anti-mouse CD25 or FITC-labeled anti-mouse CD69 (all BD Bioscience, Heidelberg, Germany). For analysis of T cell viability, cells were stained for 20 minutes with FITC-labeled anti-mouse Annexin V (BD Bioscience, Heidelberg, Germany) and propidium iodide (PI). All antibodies were titrated for optimal staining. Flow cytometry analysis was performed using a FACSCalibur® system (BD Biosciences, Heidelberg, Germany) and results were analyzed using CellQuest Pro Software (BD Bioscience, Heidelberg, Germany).
Determination of supernatant glutamate levels
Supernatant glutamate levels were determined at different time points of in vitro stimulation of WT CD8+ T cells and OT-I cells using a commercially available enzymatic determination kit (Glutamine-Glutamate Kit, Sigma-Aldrich, München, Germany) following manufacturer’s instructions. Background glutamate levels were determined in the absence of cells and subtracted from those obtained during incubation and stimulation of cells. All pharmacological blockers used did not interfere with measurements of fixed concentrations of glutamate.
Quantitative real-time PCR
For analysis of glutaminase-, vesicular (V-type) proton ATPase-, and vesicular glutamate transporter (VGluT)1-3-mRNA expression regulation, RNA was purified using Trizol reagent (Invitrogen, Carlsbad, CA, USA) and complementary DNA (cDNA) synthesis was performed using a standard protocol with random hexamer primers (all reagents were purchased from Applied Biosystems, Foster City, CA, USA) from WT CD8+ T cells before and after three days of anti-CD3/CD28 bead-stimulation. cDNA was used in quantitative real-time PCR (qRT-PCR) assays with specific TaqMan® primers and probes for murine glutaminase (Mm01257297_m1), V-type proton ATPase (Mm01222963_m1), VGluT1 (Mm00812886_m1), VGluT2 (Mm00499876_m1) and VGluT3 (Mm00805413_m1, all Applied Biosystems, Foster City, CA, USA) as well as primers for 18sRNA (Hs_4319413E; Applied Biosystems, Foster City, CA, USA) as endogenous control. qRT-PCR was performed according to the manufacturer’s protocol.
Neuronal cell culture and glutamate excitotoxicity assays
Neuronal cell cultures were obtained from WT C57BL/6 mice embryos (E18) following previously described protocols . Pregnant mice were killed by cervical dislocation and embryos were removed and transferred into warmed Hank’s balanced salt solution (HBSS, Gibco, Darmstadt, Germany). After preparation of hippocampi, tissue was collected in 5 ml of 0.25% trypsin in HBSS. After five minutes of incubation at 37°C, tissue was washed twice with HBSS and dissociated in 1 ml of neuronal medium (10% 10× modified Earl’s medium (MEM), 0.2205% sodium bicarbonate, 1 mM sodium pyruvate, 2 mM L-glutamine, 2% B27 supplement (all Gibco, Darmstadt, Germany), 3.8 mM glucose (Merck, Darmstadt, Germany), 1% penicillin/streptomycin (Biochrom AG, Berlin, Germany)) by triturating with fire polished Pasteur pipettes of decreasing tip diameter. Neurons were diluted in neuronal medium and plated at a density of 60,000 cells/cm2 on poly-D-lysine (Sigma-Aldrich, München, Germany) coated cover-slips placed in four-well plates (Nunc, Roskilde, Denmark). All neuronal cell cultures were maintained at 37°C and 5% CO2 for up to five to seven days prior to experiments. The neuronal cell culture system exhibited a purity of about 80% .
After experiments, neurons were fixed with 4% paraformaldehyde (PFA, Merck, Darmstadt, Germany), washed three times with 10 mM PBS, and incubated for seven hours at 4°C in 10 mM PBS containing 5% BSA, (Sigma-Aldrich, München, Germany), 0.2% Triton X-100 (Sigma-Aldrich, München, Germany) and 1% normal goat serum. Slices were then incubated with antibodies to NeuN (1:1,000, Chemicon, Billerica, MA, USA) or MAP2 a/b (1:200, Abcam, Cambridge, UK) and activated caspase-3 (1:400, Cell Signaling, Boston, MA, USA) overnight at 4°C. Secondary antibodies were Alexa Fluor 488-coupled goat anti-mouse IgG (Invitrogen, Carlsbad, CA, USA) and Cy3-coupled goat anti-rabbit IgG (Dianova, Hamburg, Germany) (one hour, RT). Negative controls were obtained by either omitting the primary or secondary antibody and gave no signal (data not shown). Finally, cultures were washed three times and subsequently covered with ProLong Gold Antifade Reagent with DAPI (Invitrogen, Carlsbad, CA, USA). Pictures were collected by immunofluorescence microscopy (Axiophot; Zeiss, Oberkochen, Germany).
Analysis of excitotoxic neuronal cell damage was performed via two distinct experimental approaches: (i) cultured neurons were incubated for 30 minutes with standard artificial cerebrospinal fluid (ACSF, for composition see below) supplemented with different concentrations of L-glutamate (Sigma-Aldrich, München, Germany) followed by a washout for 30 minutes with standard ACSF. The proportion of neurons showing focal swellings (beading ) was calculated as a percentage of all MAP2+ cells; (ii) cultured neurons were incubated for six hours with standard ACSF (for composition see below) supplemented with or without L-glutamate (Sigma-Aldrich, München, Germany) at a concentration of 100 μM. The proportion of neurons staining positive for activated caspase-3 was calculated as a percentage of all NeuN+ cells.
Preparation of acute brain slices and co-culture experiments with CD8+T cells
Six- to eight-week old ODC-OVA mice  were anesthetized with isoflurane and decapitated, the brain was removed and placed in ice-cold saline containing: sucrose, 200 mM; piperazine-N,N’-bis(2-ethanesulfonic acid) (PIPES), 20 mM; KCl, 2.5 mM; NaH2PO4, 1.25 mM; MgSO4, 10 mM; CaCl2, 0.5 mM; dextrose, 10 mM; pH 7.35, adjusted with NaOH. Slices were prepared as 300 μm coronal sections on a vibratome . For incubation with CD8+ T cells, slices were transferred each into a well of a 12-well plate containing standard ACSF containing (mM): NaCl, 125 mM; KCl, 2.5 mM; NaH2PO4, 1.25 mM; NaHCO3, 24 mM; MgSO4, 2 mM; CaCl2, 2 mM; dextrose, 10 mM; pH adjusted to 7.35 by bubbling with a mixture of 95% O2 and 5% CO2. Each slice was incubated with 5 × 105 activated OT-I T cells for six hours in the absence and presence of MK801 (10 μM) and NBQX (30 μM). Afterwards slices were harvested and embedded using OCT compound tissue-tek (Sakura Finetek Europe, Zoeterwoude, Netherlands) and frozen in liquid nitrogen. Ten micrometer coronal cryo-sections were obtained using a cryostat (Leica CM 1950, Wetzlar, Germany).
Immunofluorescence-staining and -microscopy
Immunohistochemical staining was performed on 10 μm coronary sections. For double labeling, slices were postfixated in 4% PFA for ten minutes and afterwards incubated in blocking solution (PBS containing 5% BSA, 1% normal goat serum and 0.2% Triton X-100). Slices were then incubated simultaneously with antibodies to NeuN (1:1,000, Chemicon, Billerica, MA, USA) and activated caspase-3 (1:400, Cell Signaling, Boston, MA, USA) overnight at 4°C. Secondary antibodies were Alexa Fluor 488-coupled goat anti-mouse IgG and Cy3-coupled goat anti-rabbit IgG. Negative controls were obtained by either omitting the primary or secondary antibody and gave no signal (data not shown). For quantification of cell densities, sections were examined with an Axiophot2 microscope (Zeiss, Oberkochen, Germany) with a CCD camera (Visitron Systems, Tuchheim, Germany). Cell densities were determined in preselected fields within the neocortex.
L-aminoadipic acid (L-AAA), 4-(2-butyl-6,7-dichloro-2-cyclopentylindan-1-on-5-yl)oxybutyric acid (DCPIB), L-β-threo-benzyl-aspartate (TBOA), carbenoxolone (carben.), pyridoxal phosphate-6-azo(benzene-2,4-disulfonic acid) (PPADS), (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine maleate (MK801) and 2,3-dihydroxy-6-nitro-7-sulphamoyl-benzo(F)quinoxaline (NBQX; all Sigma-Aldrich, München, Germany) were dissolved in H2O or DMSO as applicable and frozen as aliquots for further use. The solvent solution in the final experimental solution did not exceed 0.1%.
All results are presented as mean ± standard error of the mean (SEM). Statistical analysis was performed using student’s t-test for normally distributed samples or Mann–Whitney test for not-normally distributed datasets . A Bonferroni-corrected ANOVA was used in case of multiple comparisons. Analysis was performed using the Sigma Plot 11.0® software (Systat Software Inc., Erkrath, Germany). P-values < 0.05 were considered statistically significant (indicated as *) and P-values < 0.01 were considered highly statistically significant (indicated as **).
CD8+T cells release glutamate by a TCR-stimulus-dependent mechanism
As glutamate has important roles in the intermediary metabolism, it is present at millimolar concentrations in the cytosol of most cell types . Cytosolic glutamate may stem from transamination of α-ketoglutarate by glutamate dehydrogenase or from deamination of glutamine by glutaminase . Consistent with predominant synthesis of intracellular glutamate from deamination of glutamine, supernatant glutamate levels were significantly reduced following stimulation of CD8+ T cells for 72 hours in glutamine-free medium (relative supernatant glutamate: bead-stimulation for 72 hours in glutamine-containing medium at a bead-to-cell ratio of 1:1: 1.0 ± 0.01, bead-stimulation for 72 hours in glutamine-free medium at a bead-to-cell ratio of 1:1: 0.20 ± 0.05, P = 0.003, n = 3 mice, experiments performed in triplicates; Figure 1C).
To address the mechanism of glutamate release, we stimulated CD8+ T cells with anti-CD3/CD28-coated beads at a bead-to-cell ratio of 1:1 in the presence and absence of a variety of blockers of non-vesicular glutamate liberation (Figure 1C): L-aminoadipic acid (L-AAA, 2.5 mM) blocking the system Xc-glutamate-cystine exchanger (Takeuchi et al., 2006), 4-(2-butyl-6,7-dichloro-2-cyclopentylindan-1-on-5-yl)oxybutyric acid (DCPIB, 5 μM) blocking volume-sensitive anion channels , L-β-threo-benzyl-aspartate (TBOA, 500 μM) blocking excitatory amino acid transporters (EAATs; ), carbenoxolone (carben, 10 μM) blocking connexin hemi-channels  and pyridoxal phosphate-6-azo(benzene-2,4-disulfonic acid) (PPADS, 100 μM) blocking P2× receptors . After 72 hours of stimulation in the presence of each blocker, cell viability was controlled via flow cytometry for annexin V and propidium iodide (PI) uptake. As a consequence of cytotoxic effects, the concentration of carbenoxolone was reduced from 100 μM  to 10 μM and that of DCPIB was reduced from 20 μM  to 5 μM. Under these experimental conditions the fraction of viable annexin V- PI- cells was always > 90% (Additional file 1: Figure S1D). The presence of blockers did not affect the activation status of CD8+ T cells after 72 hours of stimulation as under all conditions the fraction of activated CD8+ CD25+ T cells was > 95% (Additional file 1: Figure S1C).
None of the tested blockers of non-vesicular glutamate release mechanisms significantly reduced supernatant glutamate levels after 72 hours of CD8+ T cell bead-stimulation (relative supernatant glutamate: bead-stimulation for 72 hours: no blocker: 1.0 ± 0.01; TBOA: 0.92 ± 0.05, P = 0.129; PPADS: 1.03 ± 0.05, P = 0.325; DCPIB: 0.91 ± 0.04, P = 0.296; carbenoxolone: 0.96 ± 0.02, P = 0.312; n = 3 mice respectively, experiments performed in triplicates; Figure 1C). Moreover, L-AAA caused a significant increase of supernatant glutamate levels (relative supernatant glutamate: bead-stimulation for 72 hours: no blocker: 1.0 ± 0.01; L-AAA: 1.42 ± 0.06, P = 0.01; n = 3 mice respectively, experiments performed in triplicates; Figure 1C), consistent with a predominant role of the system Xc-glutamate-cystine exchanger over EAATs for (re-)uptake of extracellular glutamate during bead-stimulation of CD8+ T cells.
As we were unable to unambiguously identify a non-vesicular mechanism of glutamate release from stimulated CD8+ T cells, we studied the expression regulation of genes essential for vesicular glutamate release during anti-CD3/CD28-bead stimulation of WT CD8+ T cells (Figure 1D). Quantitative real-time qRT-PCR analysis revealed an up-regulation of expression levels of glutaminase (3.3 ± 0.9 fold) responsible for the cytosolic conversion of glutamate from glutamine . Moreover, vesicular proton ATPase (3.6 ± 0.1 fold), required for vesicle acidification and vesicular glutamate transporters (VGluT 1: 77.0 ± 12.0 fold; VGluT2: 12.6 ± 7.2 fold; VGluT3: 5.2 ± 1.2 fold; n = 3 mice, all experiments performed in triplicates) required for uptake of cytosolic glutamate into vesicles  showed significant expression regulation (Figure 1D). These findings point towards vesicular release as the predominant mechanism of glutamate liberation by polyclonally activated CD 8+ T cells.
Antigen-dependent glutamate release from CD8+T cells varies with repetitive stimulation
During repetitive antigen-stimulation, CD8+ T cells with strong IL-2 receptor α (CD25) expression represent a population of rather short-lived effector cells, whereas those with low CD25 expression form a population of rather long-lived memory cells . To examine an effect of the CD8+ T cell activation status on the rate of glutamate release, splenocytes from OT-I mice were repetitively stimulated by incubation for time intervals of 72 hours with OVA-peptide257-264 (SIINFEKL; 1 nM) and IL-2 (500 IU/ml) for a total of 15 days. Single CD8+ T cell glutamate secretion rates were determined from the supernatant glutamate concentrations and cell numbers at the end of each stimulation interval (see Methods; Figure 2D). In parallel, flow cytometry was repetitively performed for activation markers CD69 and CD25 (Figure 2C). CD69 expression levels peaked after 12 hours of stimulation and went back to baseline levels afterwards (CD69: mean fluorescence intensity (MFI): day 0: 5.6 ± 4.2, day 0.5: 20.6 ± 3.2, P = 0.015). In contrast, both CD25 expression levels and single CD8+ T cell glutamate secretion rates increased during the two stimulation intervals, peaked at day 6, and subsequently decreased back towards baseline levels (CD25: mean fluorescence intensity (MFI): day 0: 2.5 ± 1.2, day 6: 43.3 ± 12.5, P = 0.002; glutamate secretion rate: day 3: 3.8 ± 1.8 fmol/h/cell, day 6: 41.1 ± 16.0 fmol/h/cell, P = 0.034; Figure 2C,D) suggesting glutamate liberation as a possible effector mechanism of antigen-activated CD8+ T cells.
Antigen-dependent glutamate release from CD8+ T cells causes excitotoxic neuronal cell death during an oligodendrocyte-directed CD8+T cell attack in the CNS gray matter
Glutamate release from activated CD8+ T cell might represent an effector mechanism in autoimmune CNS inflammation due to the ubiquitous expression of synaptic and extrasynaptic ionotropic glutamate receptors in neurons , which renders them highly sensitive to excitotoxic cell death. Moreover, encephalitogenic CD8+ T cells are known to migrate through the CNS parenchyma making short-lasting direct contacts with parenchymal cells in search of their cognate antigen. Upon antigen-recognition, CD8+ T cells make long-lasting contacts with their target cells and exert direct and collateral [4, 7, 12] cytotoxic effects. In the intact CNS parenchyma, glutamate is efficiently cleared from the extracellular space through (re-)uptake by high-affinity glial and neuronal excitatory amino acid transporters (EAATs; ). Thus, relevant excitotoxic effects of CD8+ T cell-mediated glutamate liberation can only be studied in the intact CNS parenchyma.
Excitotoxic neuronal cell death is a consequence of excessive stimulation of ionotropic glutamate receptors and can be effectively inhibited by MK801 (blocking NMDA-receptors at a concentration of 10 μM;  and NBQX (blocking AMPA-/kainate-receptors at a concentration of 30 μM; ).
To exclude relevant self-killing of OT-I T cells due to OVA-peptide257-264 loading of their MHC I molecules, we used EG-7 cells as targets, which constitutively present OVA-peptide257-264 in the context of MHC I  and obtained the same results (EG-7 cells + OT-I T cells: 259 042 ± 24 119 cpm; EG-7 cells + OT-I T cells + MK801/NBQX: 199 698 ± 25 930 cpm, P = 0.143, n = 3 mice, experiments performed in triplicates for all experimental groups, data not shown). Hence, glutamate receptor blockers did not impact the killing efficacy as a measure of migration, target cell recognition and exertion of effector functions of activated CD8+ T cells. They can thus be used to study the impact of CD8+ T cell-mediated glutamate liberation on excitotoxic neuronal cell death within the intact CNS parenchyma.
CD8+ T cell-mediated neuronal cell death is a relevant pathogenic mechanism in a variety of inflammatory CNS disorders . CD8+ T cell cytotoxicity within the CNS is predominantly mediated via two independent pathways depending on the strength of the antigen-signal (that is, the number of antigen-peptide loaded major histocompatibility complex class I (pMHC I) molecules and the affinity of the CD8+ T cell receptor (TCR) complex to the pMHC I complex) delivered by the glial or neuronal target cell [31, 32]. Strong antigen-signals promote killing via CD8+ T cell-mediated perforin and granzyme exocytosis and perforin-dependent delivery of granzymes into the target cell, whereas weak antigen-signals favor killing via interaction of CD8+ T cell-derived Fas-ligand (FasL) with the Fas-receptor (Fas) on the target cell surface [31–33]. Moreover, CD8+ T cells are able to contribute to inflammatory tissue damage by release of proinflammatory cytokines such as IFNγ and tumor necrosis factor-(TNF)α . These CD8+ T cell effector mechanisms impair electrical signaling  and cause cell death of neurons as direct and 'collateral’ target cells [4, 7].
Glutamate excitotoxicity is known to contribute to neuronal damage in autoimmune CNS inflammation [35–37]. Under inflammatory conditions, glutamate is known to be released from microglia and astrocytes [13, 20].
We provide evidence of glutamate release as another crucial effector pathway of CD8+ T cells leading to 'collateral’ excitotoxic neuronal degeneration during an oligodendrocyte-directed CD8+ T cell attack in the CNS gray matter. Following TCR stimulation, CD8+ T cells acquire the molecular repertoire for vesicular glutamate release: (i) they upregulate expression of glutaminase required to generate glutamate via deamination of glutamine and (ii) they upregulate expression of vesicular proton-ATPase and vesicular glutamate transporters required for filling of vesicles with glutamate. In contrast, there was no evidence for non-vesicular mechanisms of glutamate liberation. Furthermore, CD25high CD8+ T effector cells exhibit higher estimated single cell glutamate release rates than CD25low CD8+ T memory cells underpinning glutamate liberation as a possible effector mechanism of antigen-activated CD8+ T cells entering the CNS. Moreover, activated oligodendrocyte-reactive CD25high CD8+ T effector cells cause 'collateral’ neuronal cell death within intact cortical gray matter of acute brain slices which can partially be blocked by a combination of ionotropic glutamate receptor antagonists. Hence, glutamate liberation by oligodendrocyte-reactive CD8+ T cells is capable of eliciting excitotoxic cell death of neurons despite glutamate reuptake by glia and neurons in the intact CNS gray matter . Encephalitogenic CD8+ T cells (in contrast to CD4+ T cells) make close contacts with their target cells in the CNS  and release proinflammatory cytokines which may reinforce local glutamate excitotoxicity: TNFα is known to reduce the expression of EAATs and detoxifying enzymes in glia cells limiting their glutamate uptake capacity [38, 39]. IFNγ has been shown to exert neurotoxicity by gating a calcium-permeable complex of the IFNγ receptor and AMPA glutamate receptor in neurons . Moreover, neuron-reactive CD8+ T cells which directly contact neurites induce cytoskeleton breaks with adjacent neuritic spheroids  closely resembling neuronal beading upon exposition to glutamate . Importantly, it has been demonstrated that cell death in mature but not immature cultured neurons treated with cytotoxic secretory granules isolated from CD8+ T cells can be inhibited by blockers of ionotropic (NMDA- but not AMPA-) but not metabotropic glutamate receptors . As in our case, this effect was neuron-specific as glutamate receptor blocker failed to protect glutamate-insensitive lymphoma cells from secretory granule-induced cytotoxicity . Moreover, blocking of neuronal glutamate reuptake did not significantly enhance killing of neurons elicited by secretory granules, and relevant glutamate concentrations could not be detected in the culture supernatant. This refutes the possibility that significant neuronal (auto-)toxicity was induced by vesicular or non-vesicular release of glutamate from neurons following their perforin-mediated depolarization  upon treatment with secretory granules . However, these findings are consistent with storage of glutamate in secretory granules which together with perforin and granzymes elicits neuronal calcium overload and cell death.
Facing excess numbers of target cells, CNS-invading CD8+ T cells may kill neurons either via confined release of cytotoxic effector molecules towards the neuron or via spillover of cytotoxic effector molecules from 'leaky’ immunological synapses and non-confined release by CD8+ T cells themselves during serial and simultaneous killing of oligodendrocytes or astrocytes. In both scenarios glutamate-mediated excitotoxicity may contribute to neuronal cell death.
Artificial cerebrospinal fluid
Bovine serum albumin
Cluster of differentiation
Central nervous system
Dulbecco’s modified Eagle’s medium
Excitatory amino acid transporter
Fetal calf serum
Hank’s balanced salt solution
myelin basic protein
modified Earl’s medium
Ovalbumin-reactive CD8+ T cells
Phosphate buffered saline
Pyridoxal phosphate-6-azo(benzene-2,4-disulfonic acid)
Quantitative real-time PCR
Standard error of the mean
T cell receptor
Tumor necrosis factor
We would like to thank S Hilz for excellent technical assistance. This study was supported by a university grant (IZKF Z-3/4, University of Würzburg, Germany) to NM and HW. This work was done in partial fulfillment of the doctoral thesis of GH.
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