Induction of complement proteins in a mouse model for cerebral microvascular Aβ deposition
© Fan et al; licensee BioMed Central Ltd. 2007
Received: 30 July 2007
Accepted: 18 September 2007
Published: 18 September 2007
The deposition of amyloid β-protein (Aβ) in cerebral vasculature, known as cerebral amyloid angiopathy (CAA), is a common pathological feature of Alzheimer's disease and related disorders. In familial forms of CAA single mutations in the Aβ peptide have been linked to the increase of vascular Aβ deposits accompanied by a strong localized activation of glial cells and elevated expression of neuroinflammatory mediators including complement proteins. We have developed human amyloid-β precursor protein transgenic mice harboring two CAA Aβ mutations (Dutch E693Q and Iowa D694N) that mimic the prevalent cerebral microvascular Aβ deposition observed in those patients, and the Swedish mutations (K670N/M671L) to increase Aβ production. In these Tg-SwDI mice, we have reported predominant fibrillar Aβ along microvessels in the thalamic region and diffuse plaques in cortical region. Concurrently, activated microglia and reactive astrocytes have been detected primarily in association with fibrillar cerebral microvascular Aβ in this model. Here we show that three native complement components in classical and alternative complement pathways, C1q, C3, and C4, are elevated in Tg-SwDI mice in regions rich in fibrillar microvascular Aβ. Immunohistochemical staining of all three proteins was increased in thalamus, hippocampus, and subiculum, but not frontal cortex. Western blot analysis showed significant increases of all three proteins in the thalamic region (with hippocampus) as well as the cortical region, except C3 that was below detection level in cortex. Also, in the thalamic region (with hippocampus), C1q and C3 mRNAs were significantly up-regulated. These complement proteins appeared to be expressed largely by activated microglial cells associated with the fibrillar microvascular Aβ deposits. Our findings demonstrate that Tg-SwDI mice exhibit elevated complement protein expression in response to fibrillar vascular Aβ deposition that is observed in patients with familial CAA.
Abnormal accumulation of amyloid β peptides (Aβ) in brain is one of the major pathological characterizations of Alzheimer's disease (AD) and related disorders . Aβ, a 39–42 residue proteolytic product of the amyloid-β peptide precursor (AβPP) by β- and γ-secretase cleavages, possesses a high propensity to self-assemble into β sheet-rich fibrils . One prominent site of brain Aβ deposition is in and along the walls of cerebral blood vessels, a condition known as cerebral amyloid angiopathy (CAA), which is frequently found in AD [3, 4]. In contrast to parenchymal plaques, which can be composed of either diffuse or fibrillar deposits, cerebral vascular Aβ deposits appear to be exclusively fibrillar in nature . Several familial forms of CAA result from specific point mutations within the mid-region of the Aβ domain, which significantly enhance the fibrillogenic and cerebral vascular cytotoxic properties of Aβ [6–8].
Previously, we generated transgenic mice that express neuronally derived human AβPP, containing the Dutch-type (E693Q) and Iowa-type (D694N) familial CAA mutations, the Tg-SwDI mice . These mice were shown to develop early-onset and robust deposition of cerebral microvascular fibrillar amyloid but only diffuse parenchymal Aβ plaques. The microvascular accumulation of fibrillar Aβ is most prominent in the thalamic and subiculum regions of the brain . In these areas with the most fibrillar microvascular Aβ deposits, activated inflammatory cells, microglia and astrocytes, were also found highly enhanced . Several pro-inflammatory cytokines were shown to be elevated in Tg-SwDI mouse brains, indicating the active synthesis of inflammatory molecules by these cells [10, 11].
Another important aspect of the neuroinflammatory response in AD and CAA is the innate immune system activation, among which, complement is a key player . Complement proteins have been shown to be induced and associated with Aβ plaques in brains of AD patients and familial CAA patients as well as AD animal models, specifically those plaques containing the fibrillar form of the Aβ peptide [13–15]. The overall outcome of complement activation depends on the balance of its detrimental and beneficial effects . On one side, complement activation could induce cell lysis and cause cell death [17, 18]; on the other side, complement components such as C1q and C3b can promote the clearance of cellular debris and apoptotic cells and enhance cell survival [15, 19]. Although complement proteins are typically secreted by immune cells, in the CNS, microglia, astrocytes, and neurons have been reported being capable of producing complement components upon stimulation [20, 21]. In the current study, we investigated the expression of several native complement components in twelve months old Tg-SwDI and wild-type C57BL/6 mice, and found they were increased significantly in association with microvascular amyloid deposits and co-localized with activated microglial cells. The finding that microglial synthesis of the native complement proteins was induced suggests that early complement activation may be increased in the Tg-SwDI mouse model of cerebral microvacular Aβ deposition.
Generation of Tg-SwDI transgenic mice on a pure C57BL/6 background was recently described . These mice express low levels of human Swedish/Dutch/Iowa mutant AβPP in neurons under control of the mouse Thy1.2 promoter. The deposition of mutant Dutch/Iowa Aβ peptide starts around 3 months of age and begins to plateau at about 15 months in transgenic mice with low variation between individuals. Homozygous Tg-SwDI and nontransgenic C57BL/6 mice at 12 months of age were used in this study. All work with animals followed National Institutes of Health guidelines and was approved by Stony Brook University Institutional Animal Care and Use Committee.
At least 24 mice per genotype were euthanized at 12 months of age. After cold-phosphate buffer saline (PBS, 0.1M, pH 7.6) perfusion, brains were removed and dissected through the mid-sagittal plane. One cerebral hemisphere was immersion-fixed with 4% paraformaldehyde overnight at 4°C and subjected to increasing concentrations (10, 20, and 30%) of sucrose in PBS, then embedded in OCT compound (Sakura Finetek Inc., Torrance, CA) and snap-frozen in dry ice. Coronal sections were cut at 20-μm thickness using a Leica CM1900 cryostat (Leica Microsystems, Inc., Bannockburn, IL), and then stored in PBS with 0.02% NaN3 at 4°C.
Immunostainings were performed on sections mounted on Colorfrost/Plus slides (Fisher Scientific, Houston, TX). Antigen retrieval was performed in 1:100 antigen-unmasking solution (Vector Lab, Burlingame, CA) for 30 min at 90°C for C1q and microglia immunostaining. Nonspecific binding was prevented by incubating sections in blocking buffer (PBS containing 0.1% Triton X-100 and 2% bovine serum albumin (Sigma, St. Louis, MO) for 20 min at room temperature. Brain sections were incubated with primary antibodies diluted in blocking buffer overnight at 4°C. The following primary antibodies were used for immunostaining: mouse monoclonal antibody to glial fibrillary acidic protein (GFAP) for identification of astrocytes (1:300, Millipore, Temecula, CA); mouse monoclonal antibody 5D4 to keratan sulfate for identification of activated microglia (1:300; Seikagaku Corporation, Japan); monoclonal rat-anti-mouse C3 (1:500) and monoclonal rat-anti-mouse C4 (1:1000, Cedarlane, Burlington, NC); polyclonal rabbit-anti-mouse C1q (1:500, generous gift from Dr. Andrea J Tenner, University of California, Irvine). Biotinylated donkey anti-rat IgG and donkey anti-rabbit IgG (both at 1:200, Vector Lab), followed by Fluor590-conjugated streptavidin (1:500, Invitrogen, Carlsbad, CA) and Fluor488-conjugated anti-mouse antibody (1:500, Invitrogen) were used for immuno-detection.
The brain sections were examined using an Olympus BX60 fluorescent microscope (Olympus America Inc., Center Valley, PA) with Olympus DP10 camera (Olympus) and ASI MS-2000 motorized stage (Applied Scientific Instruments, Eugene, OR). Pictures were taken with 10x eye-piece and 40x objective.
Micro-dissected tissue was removed from the thalamic (with hippocampus) and cortical (frontal cortex) brain regions of 12-months old Tg-SwDI and non-transgenic (C57BL/6) mice (from the other hemisphere of the same animals used for histology). Half of each group were homogenized on ice in lysis buffer containing 1% SDS, 0.5% IGPEL in PBS with Complete protease inhibitor (Roche, Basel, Switzerland) at 10 ml/g tissue ratio. The protein concentration was determined using the bichinchonic acid method (Pierce, Rockford, IL). Equal amounts of protein (50 μg) were loaded, fractionated on 10% SDS-polyacrylamide gels, and subsequently electrophoretically transferred to nitrocellulose membranes (Schleicher and Schuell, Hertogen-bosch, Netherlands) in blotting buffer (25 mM Tris-HCl, pH 8.6, 192 mM glycine, and 20% methanol). Blots were washed for 15 min in PBS containing 0.05% Tween 20 (PBST), pre-incubated with blocking solution (5% non-fat milk powder in PBST), washed three times with PBST, and subsequently incubated with primary antibodies and peroxidase-labeled secondary goat anti-rat or donkey anti-rabbit antibodies (Dako, Glostrup, Denmark). Detection was performed by chemiluminescence according to the description of the manufacturer (Boehringer Mannheim, Almere, Netherlands) and exposed to Kodak (Rochester, NY) X-OMAT-R films and quantified using VersaDoc imaging system (BioRad, Hercules, CA). The molecular mass of specific bands was determined by comparing to the BenchMark pre-stained protein ladder (Invitrogen) electrophoresed on the same gels.
Real-time reverse transcriptase polymerase chain reaction
Total RNA was isolated from the tissue using RNeasy Lipid Tissue Kit (Qiagen, Hilden, Germany) as per manufacturer's instructions. Single stranded cDNA was prepared using 10 μl of total RNA using iScript cDNA Synthesis Kit (Biorad) per manufacturer's instructions. 6-carboxyfluorescein (FAM) 5' end labeled Taqman Assay on Demand primer and probe sets (Applied Biosystems, Foster City, CA) were used to assess expression levels of C1q (Mm00437836_m1) and C3 (Mm01232773_m1) for each cDNA sample. All samples were normalized against the endogenous control, TATA box binding protein (Mm00446973_m1). 500 ng of each cDNA sample was used in conjunction with 8 pmoles of each primer/probe set plus Taqman Universal Master Mix (Applied Biosystems). Each sample was analyzed in triplicates. Real-time quantitative PCR was performed on an Opticon2 (Biorad) using the following program: 95°C for 10 sec, followed by 40 cycles of denaturation, 95°C for 15 sec and anneal/elongation, 60°C for 1 min. The relative expression level of C1q or C3 was computed with respect to the mRNA expression of the endogenous control using the following formula: Relative mRNA expression = 2-(-Ct gene of interest-Ct TATA)
Biochemical and molecular data were analyzed by ANOVA single factor test at α = 0.05 significance level.
C1q, C3, and C4 were induced in Tg-SwDI mice
Complement proteins are synthesized in Tg-SwDI mouse brain
Complement proteins are expressed in activated microglia of Tg-SwDI mice
We have reported before that in Tg-SwDI mice, in spite of the low expression level of transgene encoded human AβPP, profuse fibrillar amyloid deposits were present in cerebral microvessels, likely due to the highly fibrillogenic property of the Dutch/Iowa CAA mutant Aβ peptide, with no detectable export of this Aβ into the periphery . In association with the microvascular fibrillar Aβ deposits, reactive microglia and astrocytes were found in these animals, and increased with age in parallel to the microvascular Aβ deposition . We also detected pro-inflammatory cytokines such as TNFα, IL-1β, and IL-6 to be enhanced in Tg-SwDI mice, suggesting that these activated cells are actively expressing inflammatory molecules surrounding the microvasculature [10, 11]. Previously, it has been shown that complement components are induced in AD patients and familial CAA patients as well as brains of AD animal models . In addition to the well-studied pathogen elimination by complement activation, amyloid clearance may be promoted by C1q binding to fibrillar amyloid deposits, and generation of an opsonin, the C3b fragment [15, 25]. Human studies have also shown increasing complement staining with growing presence of CAA . However, due to the inefficient binding of mouse C1q to human Aβ, complement activation is much less in mouse models for AD than in AD patients , which might explain why we did not observe the presence of late-stage complement activation products. In brain, activated microglia are the major source of complement proteins in brain, although reactive astrocytes and neurons have also been shown to express complement when stimulated [28, 29].
Here we demonstrate that early native classical complements C1q and C4, as well as C3, an important player in both classical and alternative complement pathways, are up-regulated in Tg-SwDI mouse brains. This induction was likely due to local synthesis since we have detected the increase in messenger RNA and protein levels by real-time PCR technique and Western blot, respectively. The observed differences between mRNA and protein levels of C1q and C3 were possibly due to several reasons. For example, increased levels of certain complement proteins may reflect decreased turnover and/or accumulation with amyloid deposits in the absence of increased expression. Also, the sensitivities of real-time PCR and Western blot are different depending on primers and antibodies, and the message levels may not be translated linearly to protein expression.
The immunoreactivities of C1q, C3, and C4 were cell associated and appeared to track along cerebral microvascular amyloid deposits, particularly in thalamus and subiculum (data not shown). We then investigated the cell type that expressed the complement. Double immunofluorescent labeling indicated that most complement-positive cells were activated microglia, not astrocytes. Although not all complement-positive cells overlapped with activated microglial labeling, this was possibly due to the multiple activation stages and types of microglia and one activation marker (keratan sulfate in this case [30, 31] may not reflect all activated microglial cells. These findings further support the notion that microglia in the Tg-SwDI mouse model are actively participating in the neuroinflammatory response and provide more insight into the specific reactions of microglia during disease progression as well as modulations of these reactions. In light of our recent work in which we detected improvement of memory performance in animals when microglial activation was suppressed , the effect of that inhibition on complement induction remains to be investigated to further elucidate the function of elevated complement in this model.
In summary, complement proteins C1q, C3, and C4 were induced in Tg-SwDI mice in proximity to the cerebral microvasculature. This increase in expression was primarily in thalamus and hippocampus, and also in subiculum by immunohistochemistry (data not shown), regions that contain the highest amount of fibrillar microvascular amyloid deposition. These complement proteins were locally synthesized in brain, and mostly by activated microglial cells associated with microvascular Aβ deposits.
This work is supported by NIH grants NS55118 and NS36645. The authors thank Ms. Judianne Davis for her input in modifying real-time PCR protocols and Dr. Feng Xu for helping with collecting animal brains. Special thanks to Dr. Andrea J. Tenner at University of California, Irvine for providing the anti-C1q antibody.
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