Norepinephrine enhances the LPS-induced expression of COX-2 and secretion of PGE2 in primary rat microglia
- Johannes CM Schlachetzki†1, 2,
- Bernd L Fiebich†1Email author,
- Elisabeth Haake1,
- Antonio CP de Oliveira1,
- Eduardo Candelario-Jalil3,
- Michael T Heneka4 and
- Michael Hüll1Email author
© Schlachetzki et al; licensee BioMed Central Ltd. 2010
Received: 25 October 2009
Accepted: 11 January 2010
Published: 11 January 2010
Recent studies suggest an important role for neurotransmitters as modulators of inflammation. Neuroinflammatory mediators such as cytokines and molecules of the arachidonic acid pathway are generated and released by microglia. The monoamine norepinephrine reduces the production of cytokines by activated microglia in vitro. However, little is known about the effects of norepinephrine on prostanoid synthesis. In the present study, we investigate the role of norepinephrine on cyclooxygenase- (COX-)2 expression/synthesis and prostaglandin (PG)E2 production in rat primary microglia.
Interestingly, norepinephrine increased COX-2 mRNA, but not protein expression. Norepinephrine strongly enhanced COX-2 expression and PGE2 production induced by lipopolysaccharide (LPS). This effect is likely to be mediated by β-adrenoreceptors, since β-, but not α-adrenoreceptor agonists produced similar results. Furthermore, β-adrenoreceptor antagonists blocked the enhancement of COX-2 levels induced by norepinephrine and β-adrenoreceptor agonists.
Considering that PGE2 displays different roles in neuroinflammatory and neurodegenerative disorders, norepinephrine may play an important function in the modulation of these processes in pathophysiological conditions.
Microglia, the innate immune cells of the brain, constantly screen their microenvironment and transform into an "activated" state in response to brain lesions, e.g., toxic lesions or debris and degenerating neurons [1, 2] (for review see ). Once activated, microglia secrete pro- and anti-inflammatory mediators such as cytokines and prostaglandins (for review see ). In vitro stimulation with lipopolysaccharide (LPS), the endotoxin of gram-negative bacteria, results in the secretion of neurotoxic and pro-inflammatory mediators. LPS triggers the activation of microglial cells via the anchored surface myeloid glycoprotein CD14 . CD14 has also been found to bind to amyloid-β (Aβ), the major compound found in amyloid plaques in the brains of patients with Alzheimer's disease (AD) .
LPS is known to induce the production of cyclooxygenase-2 (COX-2) enzyme in microglial cells in vitro [7–9]. COX-2 converts arachidonic acid released from membrane phospholipids to prostaglandin (PG) H2. PGH2 is then isomerized to PGE2 by terminal prostaglandin E synthases. COX-2 has emerged as a major player in inflammatory reactions in the brain and increased COX-2 expression has been considered to contribute to neurodegeneration [10, 11]. Elevated COX-2 expression has been described in AD [12–17] and COX-2 protein content in the hippocampus of AD patients may correlate with the severity of dementia  On the other hand, COX-2 has been suggested to play a physiological role in the brain for being involved in neuronal plasticity and synaptic transmission [19, 20].
In recent years it has become evident that there may exist a crosstalk between the autonomic nervous system and the immune system during inflammation [21, 22]. The catecholamine norepinephrine (NE) is a classical neurotransmitter with suggested immunomodulatory properties. NE is released by neurons into the synaptic cleft and may exert effects on glial cells that are in close vicinity. NE binds to α- and β-adrenergic receptors, and expression of α1-, α2-, β1- and β2-adrenegic receptors have been identified on microglia [23–29].
An immunosuppressive role has been suggested for NE in vitro since it attenuates LPS-induced microglial production of tumour necrosis factor (TNF)α, interleukin (IL)-1β, IL-6 and nitric oxide in vitro [24, 30, 31]. NE has been shown to reduce microglia-induced neuronal cell death [30, 32].
Although it is known that NE has immunosuppressive properties, information of NE's effect on prostaglandins is scarce. The aim of our study was to further investigate the role of NE on eicosanoid production, namely PGE2, in primary rat microglia.
Chemicals and reagents
L-(-)-Norepinephrine (+) bitartrate salt monohydrate (A9512), LPS, the α1-adrenergic agonist L-phenylephrine hydrochloride and the β2-adrenergic agonist terbutaline hemisulfate salt were obtained from Sigma-Aldrich (Taufkirchen, Germany). ICI 118,551, a selective β2-antagonist, and CGP 20712A, a selective β1-antagonist, the α2-receptor agonist clonidine, the α1-receptor antagonist nicergoline, and the β1-/β2-receptor agonist dobutamine were obtained from Tocris (distributed by Biotrend, Cologne, Germany).
Primary microglial cell cultures were established from cerebral cortices of one-day-old neonatal Wistar rats as previously described [7, 33]. Briefly, forebrains were minced and gently dissociated by repeated pipetting in Hank's balanced salt solution. Cells were collected by centrifugation, resuspended in Dulbecco's modified Eagle's medium containing 10% fetal calf serum and antibiotics and cultured on 10-cm cell culture dishes (Falcon, 5 × 105 cells/plate) in 5% CO2 at 37°C. Medium was prepared taking extreme care to avoid LPS contamination . Floating microglia were harvested from 10- to 14-day-old mixed (astrocyte-microglia) primary cultures and re-seeded into 35-mm cell culture dishes in fresh complete medium to give pure microglial cultures (2 × 104 cells/dish). Microglial cultures were washed 1 h after seeding to remove non-adherent cells. The purity of the microglial culture was >98% as previously determined by immunofluorescence and cytochemical analysis .
RNA extraction and RT-PCR analysis
Total RNA was isolated using the guanidine isothiocyanate method . Two μg of total RNA was reverse transcribed using M-MLV reverse transcriptase and random hexamers (Promega, Mannheim, Germany). One μl of the resulting cDNA was amplified using Taq DNA polymerase (Promega), dNTPs (Invitek, Berlin, Germany) and primers specific for rat COX-1 (forward, 5'-CGG CCT CGA CCA CTA CCA ATG-3'; reverse, 5'-TGC GGG GCG GGA ATG AAC T-3', annealing temperature 60°C, 30 cycles, amplicon size: 426 bp); rat COX-2 (forward: 5' TGC GAT GCT CTT CCG AGC TGT GCT 3', reverse: 5' TCA GGA AGT TCC TTA TTT CCT TTC 3', annealing temperature 55°C, 35 cycles, amplicon size: 479 bp); rat S12 (forward: 5'-ACG TCA ACA CTG CTC TAC A-3', reverse: 5'-CTT TGC CAT AGT CCT TAA C-3', 56°C, 30 cycles, amplicon size: 312 bp), that were designed using Primer Select software (DNA Star Inc., Madison, WI) and synthesized through an in-house facility (Dr. Gabor Igloi, Institute for Biology III, Freiburg, Germany). All PCR amplifications included a final 10-min extension at 72°C. The products were analyzed on a 2% agarose gel. Contamination by genomic DNA was identified by substituting total RNA instead of cDNA in the reaction mixture using S12 primers.
Western blot analysis
After the respective experimental set up, microglial cells were washed with phosphate-buffered saline (PBS) and lysed in 1.3 × SDS- (sodium dodecyl sulfate-) containing sample buffer without DTT or bromophenol blue containing 100 μM orthovanadate . Lysates were homogenized by repeated passage through a 26-gauge needle. Protein contents were measured using the bicinchoninic acid (BCA) method (kit obtained from Pierce, distributed by KFC Chemikalien, München, Germany). Bovine serum albumin (BSA) was used as a protein standard at concentrations ranging from 0.2 μg/μl to 4 μg/μl; the optical density was read at 570 nm using a microplate reader. Before electrophoresis, bromophenol blue and DTT (final concentration, 10 mM) were added to the samples. For COX-1 and COX-2 immunoblotting, 30 to 50 μg of protein from each sample was subjected to SDS-PAGE (polyacrylamide gel electrophoresis) on a 10% gel under reducing conditions. Proteins were then transferred onto a polyvinylidene fluoride (PVDF) membrane (Millipore, Bedford, MA, USA) by semi-dry blotting. The membrane was blocked for 1 or 2 h at room temperature using Rotiblock (Roth, Karlsruhe, Germany) or 5% blocking milk (BioRad, München, Germany), before the overnight incubation at 4°C with the primary antibody. Primary antibodies were goat anti-COX-1 goat (M-20, Santa Cruz, Heidelberg, Germany) and anti-COX-2 diluted 1:500 in Tris-buffered saline (TBS) containing 0.1% Tween 20 (Merck, Darmstadt, Germany) and 1% bovine serum albumin (BSA) and rabbit anti-actin diluted 1:5000 (Sigma, St. Louis, MO, USA). After extensive washing (three times for 15 min each in TBS containing 0.1% Tween 20), proteins were detected with horseradish peroxidase (HRP)-coupled rabbit anti-goat IgG (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA, 1:100,000) or HRP-coupled donkey anti-rabbit (GE Healthcare, Freiburg Germany, 1:25,000) using chemiluminescence (ECL) reagents (GE Healthcare). All western blot experiments were carried out at least three times.
Enzyme immunoassay (EIA)
Supernatants were harvested, centrifuged at 10,000 × g for 10 min, and levels of prostaglandin E2 in the media were measured by enzyme immunoassay (EIA) (Assay design, distributed by Biotrend, Cologne, Germany and Cayman Chemicals, Ann Arbor, MI, USA, respectively) according to the manufacturer's instructions. Standards from 39 to 2500 pg/ml were used; sensitivity of the assay was 36.2 pg/ml.
At least three independent experiments were used for data analysis. Original data were converted into %-values of LPS control and mean ± S.E.M. were calculated. Values were compared using t-test (two groups) or one-way ANOVA with post-hoc Student-Newman-Keuls test (multiple comparisons). Differences were considered statistically significant when p < 0.05.
NE enhances LPS-induced production of COX-2 mRNA and protein in primary rat microglial cells
Next, we investigated whether our findings on the protein level were due to increased mRNA levels, using qualitative PCR. COX-2 mRNA was marginally detectable in unstimulated microglial cells. In contrast to the finding that NE alone did not influence COX-2 protein levels, a potent induction of COX-2 mRNA expression was observed after 2 h stimulation with NE alone, which resulted in a dose-response upregulation starting with 1 nM that peaked at 10 μM NE (Fig. 1C). Addition of LPS, 10 ng/ml, further enhanced the expression of COX-2 mRNA (Fig. 1D). Thus, in agreement with the effects observed on COX-2 protein levels, we found an increase in COX-2 mRNA when microglial cells were stimulated by both LPS plus NE.
COX-2 mRNA upregulation was already observed at 30 min upon LPS plus NE treatment and reached its maximum by 1 h. In LPS-only treated microglial cells COX-2 mRNA expression levels reached a maximum and the same expression level as LPS plus NE after 6 h. In LPS only and LPS plus NE, COX-2 level did not further increase and stayed at the same level after 24 h. Interestingly, NE alone also led to an increase in COX-2 mRNA expression by 30 min and was still observed after 8 h (Fig. 2C).
NE increases release of PGE2 by LPS-stimulated primary microglial cells
We observed a significant increase in the secretion of PGE2 after 8 h of stimulation with the combination of LPS (10 ng/ml) and NE (1 μM) compared to unstimulated cells (p < 0.05). At 24 h, LPS plus NE also showed a significant elevation of PGE2 levels compared to treatment with LPS alone (p < 0.001). The enhancement of PGE2 induced by NE in LPS-stimulated microglia, but not by NE alone, confirms the previous results in which NE in combination with LPS further increased COX-2 immunoreactivity whereas NE alone did not increase COX-2 protein levels.
The NE-induced increase in COX-2 is mediated by β-adrenoreceptors
Finally, we treated LPS-stimulated microglial cells with both β-adrenergic receptor agonists and antagonists (Fig. 4C). As shown, ICI 118,551 and CGP 20712A reduced COX-2 expression in LPS- plus dobutamine-stimulated cells. After stimulation with the β2-selective agonist terbutaline plus LPS, addition of the β1-receptor antagonist CGP completely abolished COX-2 expression. Low concentrations of ICI 118,551, however, did not alter COX-2 expression levels, while at higher concentrations a modest decrease was observed.
In the present study, basal COX-2 expression in non-stimulated rat primary neonatal microglia was not detectable, suggesting that either COX-2 is generated by de novo synthesis in response to applied stimuli or, alternatively, that basal levels do not reach the threshold of immunoblot detection. NE alone induced COX-2 mRNA expression but did not affect COX-2 protein synthesis. We suppose that the COX-2 mRNA induced by NE is degraded before protein synthesis is initiated. Alternatively, a second stimulus, such as LPS, is required to set off translation of COX-2.
Different kinases are important in the regulation of COX-2 in LPS-stimulated microglia , and it has been demonstrated that NE increases the activity of mitogen-activated protein kinases (MAPK)  as well as transcription factors . Kan et al. (1999) demonstrated that, in neonatal rat cardiac myocytes, NE alone increases the activity of MAPK and, although IL-1β alone does not induce the same effect, the co-addition of IL-1β and NE results in an enhanced MAPK activity in comparison to the substances alone . Salmeterol and isoproterenol, β2- and non-selective β-adrenergic receptor agonists, respectively, enhance the phosphorylation of p38 MAPK and extracellular signal-regulated kinases (ERK) in peritoneal macrophages and RAW264.7 [40, 41]. Interestingly, pretreatment with isoproterenol decreases the release of TNFα, IL-12 and NO in LPS-stimulated macrophages. On the contrary, isoproterenol reduces the release of these same mediators after PMA stimulation, indicating that the effect of isoproterenol might depend on the stimulus .
Recently, Morioka et al. (2009) demonstrated that, in rat spinal microglia, NE reduces phosphorylation of p38 MAPK, induced by ATP, via β1- and β2-receptors . The deactivation of p38 would lead to a decrease in COX-2 protein synthesis, since p38 is involved in COX-2 mRNA stabilization . As such, it is possible that the observed increase in COX-2 mRNA may be due to increased transcription or increased stability of mRNA in rat microglia by activation of certain transcription factors and/or kinases. That could explain the increase in the COX-2 mRNA with incubation of cells with NE alone, or the enhancement of COX-2 protein synthesis when associated with LPS.
On the other hand, many post-transcriptional factors contribute to the translation of mRNA, which might not be affected by NE. This could explain the lack of effect of NE on COX-2 protein synthesis. For example, it is known that LPS induces the activation of the mammalian target of rapamycin (mTOR) . Activation of mTOR induces translation of different mRNA through its downstream targets such as the ribosomal p70S6 kinase and the initiation factor 4E-binding protein 1 . Although we did not test this possibility, it seems feasible that NE per se cannot activate the machinery responsible for the translation of COX-2 in rat microglia, but potentiates protein synthesis by increasing transcription or protein stability.
As shown before, LPS, 10 ng/ml, increases COX-2 mRNA and protein expression at 4 and 8 h, respectively . Co-stimulation with NE already at low concentrations results in earlier and a markedly enhanced induction of COX-2 mRNA and protein levels. Similar to our results, other groups have also shown that NE per se does not increase protein synthesis, but drastically increases the effect induced by LPS. In peripheral blood monocytes and monocyte-derived macrophages, NE and epinephrine alone only show a minor effect on matrix metalloproteinase (MMP)-1 and MMP-9 production . However, a combination of the catecholamine with LPS further enhances the increased production of MMP-1 and MMP-9.
Next, we investigated whether the increased intracellular levels of COX-2 evoked by LPS plus NE causes elevated levels of PGE2. In our experiments, NE plus LPS further increased the levels of PGE2. Interestingly, after 24 h stimulation with NE plus LPS, COX-2 mRNA and protein levels are similar to the increase observed in LPS alone. However, the increase observed in PGE2 is about three-fold higher in LPS plus NE than with LPS alone. Thus, it is possible that the combination of LPS and NE might induce the expression or increase the activity of other enzymes involved in PGE2 synthesis, such as phospholipase A2 and/or PGE synthases.
Dependent on the experimental setting, PGE2 can exert either neuroprotective or neurodetrimental effects. Agents reducing PGE2 synthesis in in vitro and in vivo models of chronic neurodegenerative diseases like Parkinson's disease or AD have been demonstrated to be neuroprotective due to their anti-inflammatory effects [47–52]. On the other hand, exogenous PGE2 protects neurons from LPS-induced cell death by reduction of NO and reactive oxygen species . Direct administration of PGE2 into the brain has also been shown to reduce microglial activation and TNF-α expression in brain parenchyma induced by intraperitoneal LPS injection . In addition, PGE2 protects neurons in culture from different types of noxious stimuli [29, 55, 56].
Localized inflammatory responses in the brain parenchyma have been associated with the pathogenesis and progression of AD. Inhibition of neuroinflammation has been identified as a potential therapeutic target [57–60]. COX-2 expression is elevated in the AD brains [14, 18] and PGE2 is accumulated in the cerebrospinal fluid of AD patients . It was therefore reasonable to hypothesize that inhibition of COX-2 may have a therapeutic potential in AD. Despite convincing evidence in epidemiological studies on the prevention of AD through long-term treatment with non-steroidal anti-inflammatory drugs, most clinical trials have failed to show beneficial effects [62–65], suggesting that either the molecular target or the therapeutic window has been missed.
PGE2 acts on four different receptors, EP1-EP4 , of which microglia express three, namely EP1, EP2, and EP3; the latter having been exclusively detected in activated microglia . Microglial EP2 receptors are known to enhance neurotoxic activities [10, 50–52, 68]. This would suggest that enhanced secretion of PGE2 through NE might increase microglial toxicity. However, the role of PGE2 may be far more complex due to the presence of other receptor subtypes on microglia. So far, NE-mediated regulation of EP receptor expression on microglia has not been studied.
In this study, we could show that the observed effect of NE is mediated by β-adrenoreceptor agonists. This corresponds to the findings of Minghetti and Levi, who showed that the non-selective β-adrenergic agonist isoproterenol increases COX-2 protein and PGE2 synthesis in microglial cells . In our experiments, both β1- and β2-receptors seem to mediate the enhanced effect of COX-2 production. Our data also indicate a lack of involvement of α-adrenoreceptors. The use of relatively-selective β-adrenoreceptors allowed us to further confirm the participation of β-adrenoreceptors in the enhancement of LPS-induced COX-2 expression. Based on our data, both β-adrenoreceptors subtypes seem to be involved. The antagonists used in this study, CGP20712A (β1-antagonist) and ICI118,551 (β2-antagonist) are widely used to discern the role of β-adrenoreceptor subtypes [69, 70]. Terbutaline is considered a relatively selective β2-agonist, but it also binds to the β1-adrenoreceptor . As with any pharmacological agent, these compounds are not 100% selective, therefore future studies utilizing knockout animals will be needed to fully clarify the role of distinct β-adrenoreceptor subtypes.
We did not detect that stimulation of α-adrenoreceptors results in any significant increase in COX-2 protein levels. Of note, stimulation of β-adrenoreceptors increases levels of cAMP in microglial cells, mainly through activation of the β2-adrenoreceptor . Raised intracellular cAMP levels are known to suppress activation of microglial cells [54, 72, 73]. Increased PGE2 levels may in addition stimulate the microglial EP2 receptor, which is linked to cAMP formation  and thereby contribute to the inactivation of microglia. Other cell culture experiments have shown that exogenous PGE2 results in decreased levels of pro-inflammatory cytokines such as TNF-alpha and IL-12 in LPS-stimulated microglia [75–77]. In concordance with these results, increased cAMP levels via stimulation of β-adrenoreceptors inhibits the production of macrophage inflammatory protein (MIP)-1α, which is known to activate macrophages to secrete pro-inflammatory cytokines .
Our data suggest that NE has a strong effect on microglial inflammatory responses, suggesting that NE is an active modulator of microglial activation. This may be important in AD, a disease in which early loss of noradrenergic locus coeruleus (LC) neurons has been observed . Depletion of LC neurons by injection of the neurotoxin DSP4 increases the levels of inflammatory mediators like iNOS, IL-1β and IL-6 .
Our study shows that NE increases LPS-induced COX-2 expression and PGE2 secretion by primary rat neonatal microglia in vitro. These results shed new light on the role of NE in CNS inflammation. NE via COX-2 and PGE-2 induction may have a protective physiological function rather than being purely detrimental. However, further investigations in in vivo models, especially in regard to PGE2 and COX-2, may help to clarify the exact role of NE in neuroinflammation.
We thank Ulrike Götzinger-Berger and Brigitte Günter for excellent technical assistance. Antonio Carlos Pinheiro de Oliveira was supported by CAPES (Brasília/Brazil). This work was supported by KNDD (Kompetenznetz Degenerative Demenzen).
- Nimmerjahn A, Kirchhoff F, Helmchen F: Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo. Science. 2005, 308: 1314-1318. 10.1126/science.1110647.View ArticlePubMedGoogle Scholar
- Davalos D, Grutzendler J, Yang G, Kim JV, Zuo Y, Jung S, Littman DR, Dustin ML, Gan WB: ATP mediates rapid microglial response to local brain injury in vivo. Nat Neurosci. 2005, 8: 752-758. 10.1038/nn1472.View ArticlePubMedGoogle Scholar
- Hanisch UK, Kettenmann H: Microglia: active sensor and versatile effector cells in the normal and pathologic brain. Nat Neurosci. 2007, 10: 1387-1394. 10.1038/nn1997.View ArticlePubMedGoogle Scholar
- Block ML, Zecca L, Hong JS: Microglia-mediated neurotoxicity: uncovering the molecular mechanisms. Nat Rev Neurosci. 2007, 8: 57-69. 10.1038/nrn2038.View ArticlePubMedGoogle Scholar
- Wright SD, Ramos RA, Tobias PS, Ulevitch RJ, Mathison JC: CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science. 1990, 249: 1431-1433. 10.1126/science.1698311.View ArticlePubMedGoogle Scholar
- Fassbender K, Walter S, Kuhl S, Landmann R, Ishii K, Bertsch T, Stalder AK, Muehlhauser F, Liu Y, Ulmer AJ, Rivest S, Lentschat A, Gulbins E, Jucker M, Staufenbiel M, Brechtel K, Walter J, Multhaup G, Penke B, Adachi Y, Hartmann T, Beyreuther K: The LPS receptor (CD14) links innate immunity with Alzheimer's disease. Faseb J. 2004, 18: 203-205.PubMedGoogle Scholar
- Akundi RS, Candelario-Jalil E, Hess S, Hull M, Lieb K, Gebicke-Haerter PJ, Fiebich BL: Signal transduction pathways regulating cyclooxygenase-2 in lipopolysaccharide-activated primary rat microglia. Glia. 2005, 51: 199-208. 10.1002/glia.20198.View ArticlePubMedGoogle Scholar
- Minghetti L, Levi G: Induction of prostanoid biosynthesis by bacterial lipopolysaccharide and isoproterenol in rat microglial cultures. J Neurochem. 1995, 65: 2690-2698. 10.1046/j.1471-4159.1995.65062690.x.View ArticlePubMedGoogle Scholar
- Hoozemans JJ, Veerhuis R, Janssen I, van Elk EJ, Rozemuller AJ, Eikelenboom P: The role of cyclo-oxygenase 1 and 2 activity in prostaglandin E(2) secretion by cultured human adult microglia: implications for Alzheimer's disease. Brain Res. 2002, 951: 218-226. 10.1016/S0006-8993(02)03164-5.View ArticlePubMedGoogle Scholar
- Kawano T, Anrather J, Zhou P, Park L, Wang G, Frys KA, Kunz A, Cho S, Orio M, Iadecola C: Prostaglandin E2 EP1 receptors: downstream effectors of COX-2 neurotoxicity. Nat Med. 2006, 12: 225-229. 10.1038/nm1362.View ArticlePubMedGoogle Scholar
- Hewett SJ, Uliasz TF, Vidwans AS, Hewett JA: Cyclooxygenase-2 contributes to N-methyl-D-aspartate-mediated neuronal cell death in primary cortical cell culture. J Pharmacol Exp Ther. 2000, 293: 417-425.PubMedGoogle Scholar
- Bazan NG, Colangelo V, Lukiw WJ: Prostaglandins and other lipid mediators in Alzheimer's disease. Prostaglandins Other Lipid Mediat. 2002, 68-69: 197-210. 10.1016/S0090-6980(02)00031-X.View ArticlePubMedGoogle Scholar
- Colangelo V, Schurr J, Ball MJ, Pelaez RP, Bazan NG, Lukiw WJ: Gene expression profiling of 12633 genes in Alzheimer hippocampal CA1: transcription and neurotrophic factor down-regulation and up-regulation of apoptotic and pro-inflammatory signaling. J Neurosci Res. 2002, 70: 462-473. 10.1002/jnr.10351.View ArticlePubMedGoogle Scholar
- Kitamura Y, Shimohama S, Koike H, Kakimura J, Matsuoka Y, Nomura Y, Gebicke-Haerter PJ, Taniguchi T: Increased expression of cyclooxygenases and peroxisome proliferator-activated receptor-gamma in Alzheimer's disease brains. Biochem Biophys Res Commun. 1999, 254: 582-586. 10.1006/bbrc.1998.9981.View ArticlePubMedGoogle Scholar
- Pasinetti GM, Aisen PS: Cyclooxygenase-2 expression is increased in frontal cortex of Alzheimer's disease brain. Neuroscience. 1998, 87: 319-324. 10.1016/S0306-4522(98)00218-8.View ArticlePubMedGoogle Scholar
- Yokota O, Terada S, Ishizu H, Ishihara T, Ujike H, Nakashima H, Nakashima Y, Kugo A, Checler F, Kuroda S: Cyclooxygenase-2 in the hippocampus is up-regulated in Alzheimer's disease but not in variant Alzheimer's disease with cotton wool plaques in humans. Neurosci Lett. 2003, 343: 175-179.View ArticlePubMedGoogle Scholar
- Ho L, Pieroni C, Winger D, Purohit DP, Aisen PS, Pasinetti GM: Regional distribution of cyclooxygenase-2 in the hippocampal formation in Alzheimer's disease. J Neurosci Res. 1999, 57: 295-303. 10.1002/(SICI)1097-4547(19990801)57:3<295::AID-JNR1>3.0.CO;2-0.View ArticlePubMedGoogle Scholar
- Ho L, Purohit D, Haroutunian V, Luterman JD, Willis F, Naslund J, Buxbaum JD, Mohs RC, Aisen PS, Pasinetti GM: Neuronal cyclooxygenase 2 expression in the hippocampal formation as a function of the clinical progression of Alzheimer disease. Arch Neurol. 2001, 58: 487-492. 10.1001/archneur.58.3.487.PubMedGoogle Scholar
- Kaufmann WE, Worley PF, Pegg J, Bremer M, Isakson P: COX-2, a synaptically induced enzyme, is expressed by excitatory neurons at postsynaptic sites in rat cerebral cortex. Proc Natl Acad Sci USA. 1996, 93: 2317-2321. 10.1073/pnas.93.6.2317.PubMed CentralView ArticlePubMedGoogle Scholar
- Minghetti L: Cyclooxygenase-2 (COX-2) in inflammatory and degenerative brain diseases. J Neuropathol Exp Neurol. 2004, 63: 901-910.PubMedGoogle Scholar
- Tracey KJ, Czura CJ, Ivanova S: Mind over immunity. Faseb J. 2001, 15: 1575-1576. 10.1096/fj.01-0148hyp.View ArticlePubMedGoogle Scholar
- Sternberg EM: Neural regulation of innate immunity: a coordinated nonspecific host response to pathogens. Nat Rev Immunol. 2006, 6: 318-328. 10.1038/nri1810.PubMed CentralView ArticlePubMedGoogle Scholar
- Pocock JM, Kettenmann H: Neurotransmitter receptors on microglia. Trends Neurosci. 2007, 30: 527-535. 10.1016/j.tins.2007.07.007.View ArticlePubMedGoogle Scholar
- Farber K, Pannasch U, Kettenmann H: Dopamine and noradrenaline control distinct functions in rodent microglial cells. Mol Cell Neurosci. 2005, 29: 128-138. 10.1016/j.mcn.2005.01.003.View ArticlePubMedGoogle Scholar
- Mori K, Ozaki E, Zhang B, Yang L, Yokoyama A, Takeda I, Maeda N, Sakanaka M, Tanaka J: Effects of norepinephrine on rat cultured microglial cells that express alpha1, alpha2, beta1 and beta2 adrenergic receptors. Neuropharmacology. 2002, 43: 1026-1034. 10.1016/S0028-3908(02)00211-3.View ArticlePubMedGoogle Scholar
- Tanaka KF, Kashima H, Suzuki H, Ono K, Sawada M: Existence of functional beta1- and beta2-adrenergic receptors on microglia. J Neurosci Res. 2002, 70: 232-237. 10.1002/jnr.10399.View ArticlePubMedGoogle Scholar
- Prinz M, Hausler KG, Kettenmann H, Hanisch U: beta-adrenergic receptor stimulation selectively inhibits IL-12p40 release in microglia. Brain Res. 2001, 899: 264-270. 10.1016/S0006-8993(01)02174-6.View ArticlePubMedGoogle Scholar
- Chang JY, Liu LZ: Catecholamines inhibit microglial nitric oxide production. Brain Res Bull. 2000, 52: 525-530. 10.1016/S0361-9230(00)00291-4.View ArticlePubMedGoogle Scholar
- Thery C, Dobbertin A, Mallat M: Downregulation of in vitro neurotoxicity of brain macrophages by prostaglandin E2 and a beta-adrenergic agonist. Glia. 1994, 11: 383-386. 10.1002/glia.440110411.View ArticlePubMedGoogle Scholar
- Madrigal JL, Feinstein DL, Dello Russo C: Norepinephrine protects cortical neurons against microglial-induced cell death. J Neurosci Res. 2005, 81: 390-396. 10.1002/jnr.20481.View ArticlePubMedGoogle Scholar
- Dello Russo C, Boullerne AI, Gavrilyuk V, Feinstein DL: Inhibition of microglial inflammatory responses by norepinephrine: effects on nitric oxide and interleukin-1beta production. J Neuroinflammation. 2004, 1: 9-10.1186/1742-2094-1-9.PubMed CentralView ArticlePubMedGoogle Scholar
- Madrigal JL, Dello Russo C, Gavrilyuk V, Feinstein DL: Effects of noradrenaline on neuronal NOS2 expression and viability. Antioxid Redox Signal. 2006, 8: 885-892. 10.1089/ars.2006.8.885.View ArticlePubMedGoogle Scholar
- Seregi A, Keller M, Jackisch R, Hertting G: Comparison of the prostanoid synthesizing capacity in homogenates from primary neuronal and astroglial cell cultures. Biochem Pharmacol. 1984, 33: 3315-3318. 10.1016/0006-2952(84)90099-6.View ArticlePubMedGoogle Scholar
- Gebicke-Haerter PJ, Bauer J, Schobert A, Northoff H: Lipopolysaccharide-free conditions in primary astrocyte cultures allow growth and isolation of microglial cells. J Neurosci. 1989, 9: 183-194.PubMedGoogle Scholar
- Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987, 162: 156-159. 10.1016/0003-2697(87)90021-2.View ArticlePubMedGoogle Scholar
- Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970, 227: 680-685. 10.1038/227680a0.View ArticlePubMedGoogle Scholar
- de Oliveira AC, Candelario-Jalil E, Bhatia HS, Lieb K, Hull M, Fiebich BL: Regulation of prostaglandin E2 synthase expression in activated primary rat microglia: evidence for uncoupled regulation of mPGES-1 and COX-2. Glia. 2008, 56: 844-855. 10.1002/glia.20658.View ArticlePubMedGoogle Scholar
- Kan H, Xie Z, Finkel MS: Norepinephrine-stimulated MAP kinase activity enhances cytokine-induced NO production by rat cardiac myocytes. Am J Physiol. 1999, 276: H47-52.PubMedGoogle Scholar
- Speidl WS, Toller WG, Kaun C, Weiss TW, Pfaffenberger S, Kastl SP, Furnkranz A, Maurer G, Huber K, Metzler H, Wojta J: Catecholamines potentiate LPS-induced expression of MMP-1 and MMP-9 in human monocytes and in the human monocytic cell line U937: possible implications for peri-operative plaque instability. Faseb J. 2004, 18: 603-605.PubMedGoogle Scholar
- Magocsi M, Vizi ES, Selmeczy Z, Brozik A, Szelenyi J: Multiple G-protein-coupling specificity of beta-adrenoceptor in macrophages. Immunology. 2007, 122: 503-513. 10.1111/j.1365-2567.2007.02658.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Tan KS, Nackley AG, Satterfield K, Maixner W, Diatchenko L, Flood PM: Beta2 adrenergic receptor activation stimulates pro-inflammatory cytokine production in macrophages via PKA- and NF-kappaB-independent mechanisms. Cell Signal. 2007, 19: 251-260. 10.1016/j.cellsig.2006.06.007.View ArticlePubMedGoogle Scholar
- Szelenyi J, Selmeczy Z, Brozik A, Medgyesi D, Magocsi M: Dual beta-adrenergic modulation in the immune system: stimulus-dependent effect of isoproterenol on MAPK activation and inflammatory mediator production in macrophages. Neurochem Int. 2006, 49: 94-103. 10.1016/j.neuint.2006.01.009.View ArticlePubMedGoogle Scholar
- Morioka N, Tanabe H, Inoue A, Dohi T, Nakata Y: Noradrenaline reduces the ATP-stimulated phosphorylation of p38 MAP kinase via beta-adrenergic receptors-cAMP-protein kinase A-dependent mechanism in cultured rat spinal microglia. Neurochem Int. 2009, 55: 226-234. 10.1016/j.neuint.2009.03.004.View ArticlePubMedGoogle Scholar
- Ridley SH, Dean JL, Sarsfield SJ, Brook M, Clark AR, Saklatvala J: A p38 MAP kinase inhibitor regulates stability of interleukin-1-induced cyclooxygenase-2 mRNA. FEBS Lett. 1998, 439: 75-80. 10.1016/S0014-5793(98)01342-8.View ArticlePubMedGoogle Scholar
- Weichhart T, Costantino G, Poglitsch M, Rosner M, Zeyda M, Stuhlmeier KM, Kolbe T, Stulnig TM, Horl WH, Hengstschlager M, Müller M, Säemann MD: The TSC-mTOR signaling pathway regulates the innate inflammatory response. Immunity. 2008, 29: 565-577. 10.1016/j.immuni.2008.08.012.View ArticlePubMedGoogle Scholar
- Hay N, Sonenberg N: Upstream and downstream of mTOR. Genes Dev. 2004, 18: 1926-1945. 10.1101/gad.1212704.View ArticlePubMedGoogle Scholar
- Lim GP, Yang F, Chu T, Chen P, Beech W, Teter B, Tran T, Ubeda O, Ashe KH, Frautschy SA, Cole GM: Ibuprofen suppresses plaque pathology and inflammation in a mouse model for Alzheimer's disease. J Neurosci. 2000, 20: 5709-5714.PubMedGoogle Scholar
- Scali C, Prosperi C, Vannucchi MG, Pepeu G, Casamenti F: Brain inflammatory reaction in an animal model of neuronal degeneration and its modulation by an anti-inflammatory drug: implication in Alzheimer's disease. Eur J Neurosci. 2000, 12: 1900-1912. 10.1046/j.1460-9568.2000.00075.x.View ArticlePubMedGoogle Scholar
- Teismann P, Tieu K, Choi DK, Wu DC, Naini A, Hunot S, Vila M, Jackson-Lewis V, Przedborski S: Cyclooxygenase-2 is instrumental in Parkinson's disease neurodegeneration. Proc Natl Acad Sci USA. 2003, 100: 5473-5478. 10.1073/pnas.0837397100.PubMed CentralView ArticlePubMedGoogle Scholar
- Wang T, Pei Z, Zhang W, Liu B, Langenbach R, Lee C, Wilson B, Reece JM, Miller DS, Hong JS: MPP+-induced COX-2 activation and subsequent dopaminergic neurodegeneration. FASEB J. 2005, 19: 1134-1136. 10.1096/fj.04-2370com.View ArticlePubMedGoogle Scholar
- Manabe Y, Anrather J, Kawano T, Niwa K, Zhou P, Ross ME, Iadecola C: Prostanoids, not reactive oxygen species, mediate COX-2-dependent neurotoxicity. Ann Neurol. 2004, 55: 668-675. 10.1002/ana.20078.View ArticlePubMedGoogle Scholar
- Carlson NG: Neuroprotection of cultured cortical neurons mediated by the cyclooxygenase-2 inhibitor APHS can be reversed by a prostanoid. J Neurosci Res. 2003, 71: 79-88. 10.1002/jnr.10465.View ArticlePubMedGoogle Scholar
- Kim EJ, Kwon KJ, Park JY, Lee SH, Moon CH, Baik EJ: Neuroprotective effects of prostaglandin E2 or cAMP against microglial and neuronal free radical mediated toxicity associated with inflammation. J Neurosci Res. 2002, 70: 97-107. 10.1002/jnr.10373.View ArticlePubMedGoogle Scholar
- Zhang B, Yang L, Konishi Y, Maeda N, Sakanaka M, Tanaka J: Suppressive effects of phosphodiesterase type IV inhibitors on rat cultured microglial cells: comparison with other types of cAMP-elevating agents. Neuropharmacology. 2002, 42: 262-269. 10.1016/S0028-3908(01)00174-5.View ArticlePubMedGoogle Scholar
- Akaike A, Kaneko S, Tamura Y, Nakata N, Shiomi H, Ushikubi F, Narumiya S: Prostaglandin E2 protects cultured cortical neurons against N-methyl-D-aspartate receptor-mediated glutamate cytotoxicity. Brain Res. 1994, 663: 237-243. 10.1016/0006-8993(94)91268-8.View ArticlePubMedGoogle Scholar
- Cazevieille C, Muller A, Meynier F, Dutrait N, Bonne C: Protection by prostaglandins from glutamate toxicity in cortical neurons. Neurochem Int. 1994, 24: 395-398. 10.1016/0197-0186(94)90118-X.View ArticlePubMedGoogle Scholar
- Craft JM, Watterson DM, Van Eldik LJ: Neuroinflammation: a potential therapeutic target. Expert Opin Ther Targets. 2005, 9: 887-900. 10.1517/14728188.8.131.527.View ArticlePubMedGoogle Scholar
- Hoozemans JJ, Veerhuis R, Rozemuller JM, Eikelenboom P: Neuroinflammation and regeneration in the early stages of Alzheimer's disease pathology. Int J Dev Neurosci. 2006, 24: 157-165. 10.1016/j.ijdevneu.2005.11.001.View ArticlePubMedGoogle Scholar
- Hoozemans JJ, O'Banion MK: The role of COX-1 and COX-2 in Alzheimer's disease pathology and the therapeutic potentials of non-steroidal anti-inflammatory drugs. Curr Drug Targets CNS Neurol Disord. 2005, 4: 307-315. 10.2174/1568007054038201.View ArticlePubMedGoogle Scholar
- Minghetti L: Role of inflammation in neurodegenerative diseases. Curr Opin Neurol. 2005, 18: 315-321. 10.1097/01.wco.0000169752.54191.97.View ArticlePubMedGoogle Scholar
- Montine TJ, Woltjer RL, Pan C, Montine KS, Zhang J: Liquid chromatography with tandem mass spectrometry-based proteomic discovery in aging and Alzheimer's disease. NeuroRx. 2006, 3: 336-343. 10.1016/j.nurx.2006.05.002.PubMed CentralView ArticlePubMedGoogle Scholar
- Martin BK, Szekely C, Brandt J, Piantadosi S, Breitner JC, Craft S, Evans D, Green R, Mullan M: Cognitive function over time in the Alzheimer's Disease Anti-inflammatory Prevention Trial (ADAPT): results of a randomized, controlled trial of naproxen and celecoxib. Arch Neurol. 2008, 65: 896-905. 10.1001/archneur.65.12.noc80051.View ArticlePubMedGoogle Scholar
- Thal LJ, Ferris SH, Kirby L, Block GA, Lines CR, Yuen E, Assaid C, Nessly ML, Norman BA, Baranak CC, Reines SA: A randomized, double-blind, study of rofecoxib in patients with mild cognitive impairment. Neuropsychopharmacology. 2005, 30: 1204-1215. 10.1038/sj.npp.1300690.View ArticlePubMedGoogle Scholar
- Aisen PS, Schafer KA, Grundman M, Pfeiffer E, Sano M, Davis KL, Farlow MR, Jin S, Thomas RG, Thal LJ: Effects of rofecoxib or naproxen vs placebo on Alzheimer disease progression: a randomized controlled trial. Jama. 2003, 289: 2819-2826. 10.1001/jama.289.21.2819.View ArticlePubMedGoogle Scholar
- Reines SA, Block GA, Morris JC, Liu G, Nessly ML, Lines CR, Norman BA, Baranak CC: Rofecoxib: no effect on Alzheimer's disease in a 1-year, randomized, blinded, controlled study. Neurology. 2004, 62: 66-71.View ArticlePubMedGoogle Scholar
- Narumiya S, Sugimoto Y, Ushikubi F: Prostanoid receptors: structures, properties, and functions. Physiol Rev. 1999, 79: 1193-1226.PubMedGoogle Scholar
- Slawik H, Volk B, Fiebich B, Hull M: Microglial expression of prostaglandin EP3 receptor in excitotoxic lesions in the rat striatum. Neurochem Int. 2004, 45: 653-660. 10.1016/j.neuint.2004.04.007.View ArticlePubMedGoogle Scholar
- Shie FS, Montine KS, Breyer RM, Montine TJ: Microglial EP2 is critical to neurotoxicity from activated cerebral innate immunity. Glia. 2005, 52: 70-77. 10.1002/glia.20220.View ArticlePubMedGoogle Scholar
- Baker JG: The selectivity of beta-adrenoceptor antagonists at the human beta1, beta2 and beta3 adrenoceptors. Br J Pharmacol. 2005, 144: 317-322. 10.1038/sj.bjp.0706048.PubMed CentralView ArticlePubMedGoogle Scholar
- Hieble JP: Recent advances in identification and characterization of beta-adrenoceptor agonists and antagonists. Curr Top Med Chem. 2007, 7: 207-216. 10.2174/156802607779318208.View ArticlePubMedGoogle Scholar
- Sears MR, Lotvall J: Past, present and future--beta2-adrenoceptor agonists in asthma management. Respir Med. 2005, 99: 152-170. 10.1016/j.rmed.2004.07.003.View ArticlePubMedGoogle Scholar
- Caggiano AO, Kraig RP: Prostaglandin E receptor subtypes in cultured rat microglia and their role in reducing lipopolysaccharide-induced interleukin-1beta production. J Neurochem. 1999, 72: 565-575. 10.1046/j.1471-4159.1999.0720565.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Colton CA, Chernyshev ON: Inhibition of microglial superoxide anion production by isoproterenol and dexamethasone. Neurochem Int. 1996, 29: 43-53. 10.1016/0197-0186(95)00139-5.View ArticlePubMedGoogle Scholar
- Minghetti L, Nicolini A, Polazzi E, Creminon C, Maclouf J, Levi G: Prostaglandin E2 downregulates inducible nitric oxide synthase expression in microglia by increasing cAMP levels. Adv Exp Med Biol. 1997, 433: 181-184.View ArticlePubMedGoogle Scholar
- Scales WE, Chensue SW, Otterness I, Kunkel SL: Regulation of monokine gene expression: prostaglandin E2 suppresses tumor necrosis factor but not interleukin-1 alpha or beta-mRNA and cell-associated bioactivity. J Leukoc Biol. 1989, 45: 416-421.PubMedGoogle Scholar
- Pouw Kraan van der TC, Boeije LC, Smeenk RJ, Wijdenes J, Aarden LA: Prostaglandin-E2 is a potent inhibitor of human interleukin 12 production. J Exp Med. 1995, 181: 775-779. 10.1084/jem.181.2.775.View ArticlePubMedGoogle Scholar
- Levi G, Minghetti L, Aloisi F: Regulation of prostanoid synthesis in microglial cells and effects of prostaglandin E2 on microglial functions. Biochimie. 1998, 80: 899-904. 10.1016/S0300-9084(00)88886-0.View ArticlePubMedGoogle Scholar
- Hasko G, Shanley TP, Egnaczyk G, Nemeth ZH, Salzman AL, Vizi ES, Szabo C: Exogenous and endogenous catecholamines inhibit the production of macrophage inflammatory protein (MIP) 1 alpha via a beta adrenoceptor mediated mechanism. Br J Pharmacol. 1998, 125: 1297-1303. 10.1038/sj.bjp.0702179.PubMed CentralView ArticlePubMedGoogle Scholar
- Bondareff W, Mountjoy CQ, Roth M: Loss of neurons of origin of the adrenergic projection to cerebral cortex (nucleus locus ceruleus) in senile dementia. Neurology. 1982, 32: 164-168.View ArticlePubMedGoogle Scholar
- Heneka MT, Galea E, Gavriluyk V, Dumitrescu-Ozimek L, Daeschner J, O'Banion MK, Weinberg G, Klockgether T, Feinstein DL: Noradrenergic depletion potentiates beta-amyloid-induced cortical inflammation: implications for Alzheimer's disease. J Neurosci. 2002, 22: 2434-2442.PubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.