Distribution of interleukin-1 receptor complex at the synaptic membrane driven by interleukin-1β and NMDA stimulation
© Gardoni et al; licensee BioMed Central Ltd. 2011
Received: 1 December 2010
Accepted: 11 February 2011
Published: 11 February 2011
Interleukin-1β (IL-1β) is a pro-inflammatory cytokine that contributes to neuronal injury in various degenerative diseases, and is therefore a potential therapeutic target. It exerts its biological effect by activating the interleukin-1 receptor type I (IL-1RI) and recruiting a signalling core complex consisting of the myeloid differentiation primary response protein 88 (MyD88) and the IL-1R accessory protein (IL-1RAcP). This pathway has been clearly described in the peripheral immune system, but only scattered information is available concerning the molecular composition and distribution of its members in neuronal cells. The findings of this study show that IL-1RI and its accessory proteins MyD88 and IL-1RAcP are differently distributed in the hippocampus and in the subcellular compartments of primary hippocampal neurons. In particular, only IL-1RI is enriched at synaptic sites, where it co-localises with, and binds to the GluN2B subunit of NMDA receptors. Furthermore, treatment with NMDA increases IL-1RI interaction with NMDA receptors, as well as the surface expression and localization of IL-1RI at synaptic membranes. IL-1β also increases IL-1RI levels at synaptic sites, without affecting the total amount of the receptor in the plasma membrane. Our results reveal for the first time the existence of a dynamic and functional interaction between NMDA receptor and IL-1RI systems that could provide a molecular basis for IL-1β as a neuromodulator in physiological and pathological events relying on NMDA receptor activation.
Interleukin-1β (IL-1β) is a pro-inflammatory cytokine that is involved in the pathogenesis of a number of neurological disorders, possibly as a modulator of glutamatergic response . This suggestion arises from the observation that IL-1β is often over-produced in injured tissues in which there are high levels of glutamate [2–4], and this over-production has been related to the exacerbation of glutamate-driven pathological conditions [4–6]. Various mechanisms have been identified that may explain the convergence between the IL-1β and glutamatergic systems , including hyperactivation of the NMDA receptor (NMDAR). IL-1β increases the activity of hippocampal neuronal NMDARs by phosphorylating the GluN2B subunit and thus enhancing NMDA-induced neuronal death . The same mechanism is recruited in neurons as a consequence of the IL-1β released from glia by the HIV-virus glycoprotein gp120 , and underlies the pro-convulsive effect of IL-1β . Whatever mechanism may be recruited by IL-1β, the involvement of IL-1RI is suggested by the uncontested neuroprotective effect of the IL-1 receptor antagonist (IL-1ra) [4, 10].
The binding of IL-1β to IL-1RI in the immune system leads to its association with the IL-1R accessory protein (IL-1RAcP)  and the myeloid differentiation primary response protein 88 (MyD88)  to form the core of the IL-1β/IL-1R signalling complex. However, little information is currently available concerning the molecular composition of the members of the IL-1R complex, or their subcellular distribution and functional cross-talk with NMDARs in neuronal cells [13–15]. This is a major gap in our knowledge of the pathological mechanisms involving IL-1β/IL-1RI in neurons that may be relevant to therapeutic interventions in the central nervous system (CNS).
The distribution of IL-1RI, IL-1RAcP and MyD88, together with the pre- and post-synaptic markers synaptophysin and PSD-95, was investigated in different subcellular compartments purified from adult rat hippocampi by means of western blotting , and by means of confocal microscopy of primary hippocampal neurons.
The association between IL-1RI and GluN2B was confirmed by a pull-down assay based on a fusion protein of the cytoplasmic domain of IL-1RI with GST (GST-IL-1Rcd) (Figure 2B), which contained the C-terminal 369-569 aa domain of IL-1RI. As a positive control, we used a GST-PSD-95 (PDZ1-2) fusion protein that has been previously shown to bind the GluN2B subunit of NMDA receptors . Lysates from rat hippocampal neurons were applied to affinity beads and extensively washed, after which the bound material was resolved by SDS-PAGE and underwent immunoblotting analysis using an antibody raised against GluN2B. Figure 2B shows that both IL-1Rcd and PSD-95 (PDZ1-2) associated with the GluN2B subunit, thus confirming a specific association between IL-1RI and GluN2B.
As it is well known that the synaptic localisation of receptors and ion channels, together with their protein-protein interactions, are modulated in response to various stimuli, and that they undergo dynamic changes under physiological and pathological conditions [19, 20], we investigated the possibility that IL-1RI distribution and interaction with GluN2B may be dynamically modulated. Given the relationship between the IL-1β receptor complex and NMDAR, we treated primary hippocampal neurons with IL-1β, 0.05 ng/ml, for 30 min (a concentration that also enhances NMDAR activity)  or NMDA, 50 μM, in ACSF buffer : the NMDA was applied to the neurons for 10 min, after which the cells were washed and incubated for a further 20 min in ACSF buffer. We first tested whether IL1-β and/or NMDA modulated the interaction between IL-1RI and the GluN2B subunit of the NMDA receptor (Figure 2C). IL-1RI was immunoprecipitated from total lysates of primary hippocampal neurons treated or not with NMDA, 50 μM, or IL-1β, 0.05 ng/ml, and assayed for GluN2B by means of western blotting (Figure 2C). The results show that only NMDA significantly increased the interaction between IL-1RI and GluN2B (Figure 2C; p < 0.05 NMDA vs control).
The increase in IL-1RI receptors at the postsynaptic site may be due to new synthesis and delivery of receptors from the endoplasmic reticulum, or to lateral diffusion from adjacent compartments [19, 21], and this was addressed by carrying out surface expression assays using the non-cleavable, membrane-impermeable crosslinking agent BS3 . Primary hippocampal neurons were treated with IL-1β, 0.05 ng/ml, or NMDA, 50 μM, and then exposed to BS3, lysed and blotted for IL-1RI. The intracellular amount of IL-1RI was reduced by NMDA but not by IL-1β (p < 0.05, NMDA vs control; Figure 3B). The reduction in intracellular IL-1RI after NMDA exposure, together with its increase in the synaptic fraction, suggests that NMDAR activation favours the membrane insertion of new IL-1RI. Alternatively, the increase in IL-1RI in the synaptic membrane may be attributable to stabilisation of the complex with NMDAR (within the core of the PSD), which could prevent lateral movement and/or endocytosis. In either case, a new pool of receptors would be made available. On the contrary, IL-1β possibly enriches IL-1RI at post-synaptic sites, promoting its lateral translocation (i.e. membrane diffusion) from extra-synaptic sites; however, this probably does not occur within the core microdomain of the PSD, as suggested by the unchanged levels of IL-1RI associated with the NMDAR complex.
In conclusion, ours are the first findings showing a molecular interaction between IL-1RI and the GluN2B subunit of NMDAR, and suggest a new molecular mechanism by means of which IL-1β and NMDA may dynamically regulate IL-1RI at post-synaptic sites. Furthermore, NMDA-dependent activation increases the amount of IL-1RI inserted into the membrane capable of interacting with released IL-1β. This suggests a new molecular mechanism by means of which IL-1β may contribute to excitotoxicity, thus opening up new possibilities for targeted inhibition strategies that can be used in IL-1β/glutamate-driven CNS diseases.
IL-1 receptor antagonist
interleukin-1 receptor type I
IL-1R accessory protein
myeloid differentiation primary response protein 88
Triton-insoluble postsynaptic fraction.
Acknowledgements and funding
The study was supported by Progetti di Ricerca Indipendente Regione Lombardia - Iris-Biorad to BV and by PRIN2008 to MDL. We would like to thank Dr. Polentarutti for kindly providing the IL-1RI cDNA.
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