Figure 6

There was no change in the phagocytic ability of EMF-exposed N9 cells incubated in anti-TLR4 antibody. Experiments were performed at 12 h after EMF exposure or 200 ng/ml LPS treatment as described above. (A,B) Anti-TLR4 antibody (10 μg/ml) pretreatment had no effect on microglial phagocytosis. As a positive control, anti-TLR4 antibody prevented LPS-induced depression of microglial phagocytosis. Scale bar: 10 μm. (C) No significant changes in MFG-E8 expression were detected upon anti-TLR4 antibody preconditioning. *P < 0.05 vs the sham-exposed control group; ^# P < 0.05 vs the EMF-exposed group. con, control; EMF, electromagnetic field; LPS, lipopolysaccharide; MFG-E8, milk-fat globule EGF factor-8; TLR4, toll-like receptor 4.