Fig. 1
scRNA-seq analysis of immune cells during EAU and post-DMF administration. A. Representative fundus images and clinical scores of normal, EAU, and DMF-treated EAU mice. Arrows indicate inflammatory exudation and vascular deformation. Each group contained six mice. The values represent the mean ± SD. Significance was determined using Kruskal Wallis Test. B. Representative fundus hematoxylin and eosin staining plots and pathological scores of normal, EAU, and DMF-treated EAU mice. Arrows indicate retinal folding and inflammatory cell infiltration. Scale bars, 20 mm. Each group contained six mice. The values represent the mean ± SD. Significance was determined using Kruskal Wallis Test. C. Scheme of the overall study design. IRBP1 − 20: interphotoreceptor retinoid-binding protein 1–20; PTX: pertussis toxin; CFA: complete Freund’s adjuvant. D. UMAP plots of immune cell clusters from the CDLNs of all the mouse groups. E. UMAP plots of canonical markers of immune cell clusters from all the mouse groups. F. Line charts showing the percentages of major immune cell types in normal, EAU, and DMF-treated EAU mice. G-H. Volcano plots showing upregulated and downregulated DEGs of all immune cell types in the DMF-treated EAU/EAU (G) and EAU/Normal (H) comparison groups. The red and blue dots indicate upregulated and downregulated DEGs, respectively. I. Venn diagrams showing the numbers of rescue DEGs of all immune cells. The overlapping parts indicate the numbers of downregulated rescue DEGs (top) and upregulated rescue DEGs (bottom). J. Bar plot showing GO terms enriched by downregulated or upregulated rescue DEGs of all immune cells