Quantification of microglia activation. A) Distribution of microglia according to soma sizes in 4-month-old control and TGF-β1 mice fed for 2 months with control chow, or chow containing pioglitazone or ibuprofen; B) Evolution of microglia activation with age; C) Effect of anti-inflammatory drugs. "Activated microglia" were cells with somas larger than 150 μm2. The values are the means ± SD; (**) p < 0.01; (***) p < 0.001, one-way ANOVA analysis, Bonferroni post hoc test. N = 2 for 2-month-old mice, and N = 4–6 for 4 or 9-month-old mice.