Figure 5

Pharmacologic suppression of dendritic degeneration in CA1 pyramidal neurons of mouse hippocampus. Adult (6 to 8 week old) wt C57Bl/6 mice received ICV saline or ICV LPS 24 hr prior to sacrifice. Tissue sections of hippocampus and surrounding structures were processed for Golgi stain and then evaluated by Neurolucida. Data are dendritic spine density for CA1 hippocampal pyramidal neurons (n > 6 neurons for each group). Two-way ANOVA had P < 0.001 for ICV saline vs. ICV LPS, effect of drugs, and interaction. Post hoc one-way ANOVA showed that no effect of drugs in ICV saline exposed mice. Ibuprofen and α-tocopherol completely protected spine density from ICV LPS exposure (P < 0.01 compared to vehicle treated mice) while naproxen and γ-tocopherol did not significantly protect (P > 0.05).