Figure 2

Aβ inhibits oxLDL binding, uptake and degradation in microglia. Treatment of primary microglia with 20 μM fibrillar Aβ, but not revAβ, inhibits ¹²⁵I-oxLDL binding (A), cellular uptake (B) and degradation (C). Data are the mean of triplicate samples ± standard deviation, *p ≤ 0.005. (D) Microglia treated with 20 μM fibrillar Aβ fail to accumulate cholesterol ester in the presence of oxLDL. Microglia were incubated with 40 μg/ml oxLDL for 48 h in the presence and absence of 20 μM Aβ or revAβ peptide and stained with oil red O to visualize neutral lipid. Cells treated with oxLDL alone or in the presence of revAβ demonstrate the accumulation of red-stained lipid droplets in the cytoplasm. By contrast, oil red O staining is greatly reduced in oxLDL and Aβ co-treated microglia. Mag. 200X.