Figure 10

NE inhibits LPS dependent IL-1β production. (A) Microglia were incubated in medium alone (○), 1 ng/ml LPS (●), 10 μM NE (▽), or LPS plus NE (▼) and IL-1β levels were measured by ELISA at the indicated times. Data are means ± s.e.m. and are expressed as pg of IL-1β/ml. ***, P < 0.001 versus control; §§§ and §§, P < 0.001 and 0.01 versus LPS alone (n = 3); two-way ANOVA followed by Bonferroni's post hoc test. (B) Microglia were incubated in presence of 1 ng/ml LPS plus the indicated amounts of NE, and IL-1β levels measured by ELISA after 4 hours. Data are presented as % of the response to LPS alone (100% = 1160 ± 80 pg/ml). ***, P < 0.001 versus LPS alone (n = 3); one-way ANOVA followed by Dunnett's multiple comparison test.