Figure 9

NE reduces LPS-induced IkBα loss. (A) NOS2 expression requires NFkB activation. Microglia were incubated with LPS (1 ng/ml) and the indicated concentration of the selective NFkB inhibitor ZIE. Nitrite levels were measured after 24 hr incubation. **, P < 0.01 versus LPS alone. (B) Microglial were incubated with LPS or LPS and NE (1 μM), and at the indicated time points, cytosolic lysates were examined for the presence of IkBα protein by western blot analysis. The gel shown is representative of experiments done on 3 separate microglial preparations.