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Table 1 Endotoxin determination of Hb/hemin preparations

From: Hemoglobin induces inflammation after preterm intraventricular hemorrhage by methemoglobin formation

Sample Source LAL assay before EndoTrap (EU/mg) LAL assay after EndoTrap (EU/mg) LPS ELISA after EndoTrap
OxyHb In-house purified 2.48 Not detectable Not detectable
MetHb In-house purified Not tested Not detectable Not detectable
MetHb Sigma 10.29 Not detectable Not detectable
CyanHb In-house purified 3.64 Not detectable Not detectable
Hemin Porphyrin Products Not detectable Not detectable Not detectable
  1. Determination of endotoxin levels in the Hb- and hemin preparations using an Endpoint Chromogenic LAL assay and a LPS ELISA assay as described in the Methods section. The endotoxin levels were determined both before and after purification of endotoxins using EndoTrap, as described in the Methods section. The endotoxin levels are determined as EU endotoxin per mg of Hb/hemin. LPS, lipopolysaccharide; MetHb, methemoglobin; OxyHb, oxyhemoglobin.