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Table 1 Endotoxin determination of Hb/hemin preparations

From: Hemoglobin induces inflammation after preterm intraventricular hemorrhage by methemoglobin formation

Sample

Source

LAL assay before EndoTrap (EU/mg)

LAL assay after EndoTrap (EU/mg)

LPS ELISA after EndoTrap

OxyHb

In-house purified

2.48

Not detectable

Not detectable

MetHb

In-house purified

Not tested

Not detectable

Not detectable

MetHb

Sigma

10.29

Not detectable

Not detectable

CyanHb

In-house purified

3.64

Not detectable

Not detectable

Hemin

Porphyrin Products

Not detectable

Not detectable

Not detectable

  1. Determination of endotoxin levels in the Hb- and hemin preparations using an Endpoint Chromogenic LAL assay and a LPS ELISA assay as described in the Methods section. The endotoxin levels were determined both before and after purification of endotoxins using EndoTrap, as described in the Methods section. The endotoxin levels are determined as EU endotoxin per mg of Hb/hemin. LPS, lipopolysaccharide; MetHb, methemoglobin; OxyHb, oxyhemoglobin.
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Journal of Neuroinflammation

ISSN: 1742-2094