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Figure 3 | Journal of Neuroinflammation

Figure 3

From: Western-type diet modulates inflammatory responses and impairs functional outcome following permanent middle cerebral artery occlusion in aged mice expressing the human apolipoprotein E4 allele

Figure 3

Ischemia-induced brain astrogliosis is altered upon receiving a high-fat diet. (A) Glial fibrillary acidic protein (GFAP) and (B) Iba-1 immunoreactivities were quantified from the peri-ischemic area at 3 days post stroke. Example images of GFAP and Iba-1 staining in the peri-ischemic region of wild-type (WT) mice on (C, D) a normal diet (ND) and (E, F) a high-fat (HF) diet, of ApoE3-TR mice on (G, H) normal chow and (I, J) a HF diet, and of ApoE4-TR mice on (K, L) normal chow and (M, N) a HF diet, respectively. ApoE, apolipoprotein E; E3, E4 and WT, ApoE3-TR, ApoE4-TR and control mice; TR, targeted replacement. Scale bar = 50 μm. Data expressed as mean ± standard error of the mean. For quantification of GFAP immunoreactivity: WTND, n = 8; WTHF, n = 8; E3ND, n = 6; E3HF, n = 6; E4ND, n = 8; E4HF, n = 7. For quantification of Iba-1 immunoreactivity: WTND, n = 8; WTHF, n = 7; E3ND, n = 6; E3HF, n = 6; E4ND, n = 7; E4HF, n = 8. * p < 0.05 and ** p < 0.01.

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Journal of Neuroinflammation

ISSN: 1742-2094