r-PGRN treatment significantly suppresses neutrophil recruitment into the I/R brain following MCAO. (A) Representative immunohistochemical staining for myeloperoxidase (MPO) in each of the areas of interest in the sham-operation, vehicle-treated and r-PGRN-treated groups. (B) Quantification of MPO-immunoreactive cells. The number of MPO-positive cells was significantly higher in the vehicle-treated mice than in the r-PGRN-treated mice. Scale bar = 20 μm. ## P <0.01 vs. sham-operation mice; ** P <0.01 vs. vehicle-treated mice; Student's t-test. n = 4 or n = 5 for each group. I/R, ischemia-reperfusion; MCAO, middle cerebral artery occlusion; r-PGRN, recombinant-progranulin.