Figure 3
Effect of MSC treatment on GFAP + astrocytes and Iba-1 + microglia/macrophages. (A) Diagram of a coronal rat brain section showing the relationship of the lesion cavity (red) to the regions photographed (blue squares). The density of astrocytes was not significantly different after MSC treatment (B, D) (n = 6 per group). The number of microglia/macrophages (C, E) (sham: 142.7 ± 45.4 cells/mm2; PBS: 1,524.7 ± 60.1 cells/mm2; MSCs: 1,124.3 ± 104.5 cells/mm2) was significantly decreased after MSC administration at 72 h post-TBI compared with the PBS-treatment group (n = 6 per group). Data are presented as mean ± SD. Bar = 50 μm. *p < 0.05.