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Figure 3 | Journal of Neuroinflammation

Figure 3

From: In an in vitro model of human tuberculosis, monocyte-microglial networks regulate matrix metalloproteinase-1 and -3 gene expression and secretion via a p38 mitogen activated protein kinase-dependent pathway

Figure 3

p38 phosphorylation controls both matix metalloproteinsase (MMP)-1 and MMP-3 secretion in conditioned media from tuberculosis (TB)-infected monocytes (CoMTb)-stimulated microglia. Microglial cells were incubated with SB203580 for 2 hours, and then stimulated with CoMTb. (A) MMP-1 activation and secretion was analyzed at 72 hours by casein zymography (representative gel shown) and western blotting. (B) MMP-3 activity was analyzed by ELISA and western blotting. p38 inhibition suppressed CoMTb MMP-1 and MMP-3 suppression in a dose-dependent manner. Bars represent mean values ± SD of three samples, representative of at least duplicate experiments performed in triplicate. Data were analyzed by one-way analysis of variance, followed by Tukey’s multiple comparison test. * P <0.05, ** P <0.01.

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