CR1 and microglia activation are required for CX
CL1 effects. Points represent mean ± SEM of the N-methyl-d-aspartate receptor component of field excitatory postsynaptic potential (NMDA-fEPSP) slopes. Average timecourse of changes in NMDA-fEPSPs recorded in hippocampal slices. Horizontal bar, CX3CL1 application (5 nM). (A) CX3CL1-mediated effects required the presence of CX3CR1 (n = 7/3). (B) Microglia activation is necessary for CX3CL1 action. Slices pretreated for 1 h with minocycline (last 10 minutes in the graph) and then continuously superperfused. Cotreatment with CX3CL1 did not increase NMDA-fEPSPs (n = 8/2).