Figure 7
CX 3 CL1 and 5-(6-amino-2-(phenethylthio)-9H-purin-9-yl)- N -ethyl-3,4-dihydroxytetrahydrofuran-2-carboxamide (VT7) induce extracellular accumulation of d -serine. Mass spectroscopy analysis of d-serine levels in primary purified microglia or mixed glia culture medium (A,B). Cells were stimulated with CX3CL1 (10 nM) or vehicle and after 20 minutes, 2 h and 20 minutes or 4 h (as indicated) medium was collected and analyzed for d-serine content. Results are expressed as ng extracellular d-serine accumulated per mg of cellular proteins and are the mean ± SEM of eight independent experiments. (A) Shows purified microglia, (B) shows mixed glia culture medium. (C,D) Cells stimulated with VT7 (50 nM) plus adenosine deaminase (ADA, 1 U/ml) or vehicle (ADA, 1 U/ml) and samples collected as in (A,B). (C) Shows purified microglia, (D) shows mixed glia culture medium. Results are the mean ± SEM of nine experiments (C) or 12 experiments (D). White square, vehicle; black square treatment, as indicated. *P <0.05.