Figure 8

Effect of in vitro oxygen-glucose deprivation in primary rat astrocytes on peripheral neuron injury. The double-staining of ceramide and 4′,6-diamidino-2-phenylindole were detected by immunofluorescence in the different groups after primary rat astrocytes were deprived of oxygen-glucose in vitro for 3 h and 6 h, before regaining 30 min oxygen-glucose (A), or were treated with daunorubicin (DNR) for 24 h (B). (C) After primary rat astrocytes were deprived of oxygen-glucose in vitro for 6 h (before regaining 30-min oxygen-glucose), they were cocultured with primary rat neurons for 12 h and the latter were labeled with microtubule-associated protein 2 (MAP2) fluorescence. (D) After pretreatment with DNR for 24 h, astrocytes were cocultured with neurons for 12 h and then MAP2 and terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick-end labeling staining were used to detect neuronal injury. (E) After concurrent pyrrolidine dithiocarbamate (PDTC) and DNR pretreatment, astrocytes were cocultured with neurons for 12 h and the latter were labeled with MAP2 fluorescence (n = 4).