Effects of MCP-1/CCR2 inhibition on the formation of hilar-ectopic granule cells. (A) Granule cells were immunolabeled with the granule cell-specific marker Prox1 in controls, vehicle-treated SE rats, and RS102895-treated SE rats. In SE rats, Prox1 expression was observed not only in the granule cell layer but also in the hilus. Note that the number of Prox1-expressing cells in the hilus was reduced in the SE rats treated with RS102895. Arrows denote the Prox1-positive cells. Scale bar: 200 μm. (B) Confocal micrographs for Prox-1 (red) and CCR2 (green) showing a co-expression of CCR2 in Prox-1-expressing cells in the hilus at 14 days post-SE. The middle and bottom panels show projected z-stack confocal images to demonstrate colocalization of Prox1 with CCR2, as denoted by arrows. White brackets mark the magnified views of the area. Scale bar: top and middle, 50 μm; bottom, 10 μm. GCL: granule cell layer; H: hilus.