Figure 5

Confocal micrographs of Prox1-expressing progenitors (green) co-labeled with Dcx (red) in the hilus regions of control rats, vehicle-treated SE rats, and RS102895-treated SE rats on Day 14 after SE. The middle and right panels show projected z-stack confocal images to demonstrate colocalization of Prox1 with Dcx. Prox1/Dcx-expressing progenitors appeared within the SGZ in control and SE rats. Note that Prox1/Dcx-expressing progenitors appeared ectopically within the hilus after SE, and the formation of these hilar ectopic neuronal progenitors was attenuated by administration of RS102895. Arrows denote the Prox1/Dcx-positive cells. White brackets mark the magnified views of the area. The cell denoted by the asterisk is enlarged to illustrate the pattern of Prox1 and Dcx labeling. Scale bar: left and middle, 50 μm; right, 10 μm. GCL: granule cell layer; H: hilus; SGZ: subgranular zone.