IL-1β mRNA expression by in situ hybridization in the right hemisphere of lipopolysaccharide (LPS) + hypoxia-ischemia (HI)-exposed brains versus control (Ctl). Absence of IL-1β mRNA detected in neurons from Ctl brains showing pure hematoxylin staining (arrowhead) (A). High IL-1β mRNA expression (arrowhead) detected in neocortical (BI) and deep gray (BII) neurons 1 h after LPS + HI exposure (B). Mean ± standard error of the mean IL-1β mRNA relative expression in gray matter of LPS + HI animals compared to Ctl (C) (3 animals per experimental condition). Scale bar = 10 μm. Unpaired t-test with Welch correction; ****
P ≤ 0.0001.