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Figure 5 | Journal of Neuroinflammation

Figure 5

From: PD-L1 enhances CNS inflammation and infarct volume following experimental stroke in mice in opposition to PD-1

Figure 5

Absence of PD-L1 resulted in loss of suppressor T cells from spleens. Splenocytes from WT, PD-L1-/-, and PD-L2-/- mice were harvested at 96 hours post-MCAO and assessed. (A) Expression of CD8+CD122+ T cells. Data are representative of two independent experiments with spleens processed from four to five individual mice (mean ± SEM). Statistical analysis was performed with ANOVA followed by Tukey’s multiple comparison post-hoc test. Significant differences between sample means are indicated (*P ≤0.05 compared to the PD-L1-/- mice post-MCAO). Subsequently, mononuclear cells were isolated from brains of WT, PD-L1-/-, and PD-L2-/- male mice and analyzed. (B) CD8+CD122+ T cells and IL-10 production by gated CD8+CD122+ T cells. Data are representative of two independent experiments with spleens processed from four to five individual mice (mean ± SEM). Statistical analysis was performed with ANOVA followed by Tukey’s multiple comparison post-hoc test. Significant differences between sample means are indicated (#P ≤0.05 as compared to their respective left hemisphere and *P ≤0.05 as compared to the ischemic right hemisphere of PD-L1-/- mice post-MCAO). -/-, knockout; ANOVA, analysis of variance; MCAO, middle cerebral artery occlusion; PD-L1, programmed death-1 ligand 1; PD-L2, programmed death-1 ligand 2; SEM, standard error of the mean; WT, wild-type.

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