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Figure 6 | Journal of Neuroinflammation

Figure 6

From: PD-L1 enhances CNS inflammation and infarct volume following experimental stroke in mice in opposition to PD-1

Figure 6

Absence of PD-L1 led to decreased expression of CD80 on APCs in spleens post-MCAO. Splenocytes from WT, PD-L1-/-, and PD-L2-/- mice were harvested at 96 hours post-MCAO or sham-treatment and assessed for expression. CD80 expression on gated (A) CD11c+, (B) CD11b+, and (C) CD19+ cells. (D) Expression of total PD-L1 and (E) PD-L2 on leukocytes. Values represent mean numbers (± SEM) of indicated cell subsets, gated on live leukocytes (by PI exclusion) from four to five mice of each group, from two separate experiments. Statistical analysis was performed with ANOVA followed by Tukey’s multiple comparison post-hoc test. Significant differences between sample means are indicated (#P ≤0.05; ##P ≤0.01; ###P ≤0.001 as compared to their respective sham; *P ≤0.05 and **P ≤0.01 compared to the PD-L1-/- mice post-MCAO). -/-, knockout; ANOVA, analysis of variance; APC, antigen-presenting cell; MCAO, middle cerebral artery occlusion; PD-L1, programmed death-1 ligand 1; PD-L2, programmed death-1 ligand 2; PI, propidium iodide; SEM, standard error of the mean; WT, wild-type.

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