PD-L1 enhances CNS inflammation and infarct volume following experimental stroke in mice in opposition to PD-1

Table 1 Neurological deficit score distribution and median scores at various reperfusion time points following 60 minutes of middle cerebral artery occlusion (MCAO) in wild-type (WT, C57BL/6J), PD-L1 ^-/- , and PD-L2 ^-/- mice

Experimental groups	Distribution of neurological deficit scores post-MCAO
	1 hour					24 hours					48 hours					72 hours					96 hours
	0	1	2	3	4	0	1	2	3	4	0	1	2	3	4	0	1	2	3	4	0	1	2	3	4
WT (n = 11)	0	0	4	7	0	0	4	1	6	0	0	5	3	6	0	0	5	5	1	0	0	5	2	4	0
PD-L1^-/- (n = 12)	0	1	10	1	0	0	9	2	1	0	0	11	0	1	0	0	11	1	0	0	0	12	0	0	0
PD-L2^-/- (n = 12)	0	0	6	6	0	0	3	8	1	0	0	4	8	0	0	0	7	2	3	0	0	7	1	1	3
	Median neurological deficit scores post-MCAO
	1 hour ( P = 0.013)					24 hours ( P = 0.044)					48 hours ( P = 0.031)					72 hours ( P = 0.062)					96 hours ( P = 0.020)
WT (n = 11)	3					3					2					2					2
PD-L1^-/- (n = 12)	2^a					1^a					1					1					1
PD-L2^-/- (n = 12)	2.5					2					2					1					1

^a P <0.05 compared to WT. ^-/^-, knockout; MCAO, middle cerebral artery occlusion; PD-L1, programmed death-1 ligand 1; PD-L2, programmed death-1 ligand 2; WT, wild-type.

ISSN: 1742-2094