Figure 7

Immunofluorescent labeling for caspase-3 at 3 days and 14 days post injury. Representative microscopic photos show numbers of caspase-3 immunoreactive cells inside the lesion area at 3 days post injury (A), while only few cells are caspase-3 positive (arrow) in the area of or adjacent to hematoma (B). At 14 days post injury, there are casepase-3 positive cells in the lesion site but decreased in number (C); while in the area adjacent to the hematoma, caspase-3 immunoreactive cells are markedly increased (D), compared to that at 3 days. TLR4 immunoreactivity, red; IBa-1 immunoreactivity, green; Hoechest 33342, blue. L, lesion site; H, hematoma. Bar = 50 μm.