Figure 1
From: Exercise reduces activation of microglia isolated from hippocampus and brain of aged mice
Proportion of hippocampal microglia positive for MHC II in Experiment 1. (A) Representative dot-plot of microglial cells (lower right corner) isolated from the hippocampus and brain samples using a Percoll gradient from mice in Experiment 1. (B) Representative dot-plot of cells isolated from spleen samples for positive control for CD11b and CD45 labeling. (C) Average proportion of microglia isolated from the hippocampus of aged (males n=7, females n=7) and adult (males n=9, females n=9) running-wheel and control mice (aged males n=7, aged females n=7, adult males n=9, adult females n=9) from Experiment 1 that were positive for MHC II. Wheel-running significantly decreased the proportion of MHC II positive microglia in aged females, but increased the proportion in aged males and adult females. Means ± SEM. * indicates a significant difference from age- and sex-matched control mice.