Supernatant of NSPC
inhibits proliferation, and the production of IFN-γ and IL-17 by spleen cells. C57BL/6 spleen cells were stimulated with ConA in the presence of neurobasal medium (white bar, circles) or culture supernatant of NSPCIL-10 (black bar, squares) or NSPC (grey bar, diamonds). (a) Proliferation was determined by 3H-thymidine incorporation, and production of (b) IFN-γ and (c) IL-17 was assessed by ELISA. *P <0.05. Mean values are expressed with SD from three independent experiments. ConA, concanavalin A; ELISA, enzyme-linked immunosorbent assay; IFN, interferon; IL, interleukin; NSPC, neural stem/progenitor cell.