Figure 2
Altered expression of complement components in hSOD1G93Aand wild-type mice at different ages of disease progression. (A) mRNA expression of CD55 in the lumbar spinal cord of hSOD1G93A transgenic mice relative to age-matched wild-type (WT) mice at four different ages. (B) Representative western blot of CD55 with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in the lumbar spinal cord of hSOD1G93A mice (SOD1) relative to age-matched WT mice, at different ages of disease progression. (C) Protein expression of CD55 determined by semi-quantitative densitometry in the lumbar spinal cord of hSOD1G93A mice relative to age-matched WT mice at four different ages. (D) to (F) mRNA expressions of C4 (classical pathway, D), factor B (alternative pathway, E) and CD59a (regulator, F) in lumbar spinal cord of hSOD1G93A mice relative to age-matched WT mice at four different ages. (A, C, D, E, F) Dashed lines, baseline expressions in WT controls at each time point. Data expressed as mean ± standard error of the mean (n = 6 mice/group; *P <0.05, **P <0.01, ***P <0.001, Student t test).