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Figure 2 | Journal of Neuroinflammation

Figure 2

From: Excitotoxic neuronal cell death during an oligodendrocyte-directed CD8+ T cell attack in the CNS gray matter

Figure 2

Upon antigen-dependent stimulation CD8 + T effector cells exhibit higher estimated single cell glutamate release rates than CD8 + T memory cells. (A, B) Supernatant glutamate (A ) and IFNγ (B ) concentrations determined after 72 hours of stimulation of splenocytes from OT-I mice bearing a transgenic ovalbumin (OVA)-reactive TCR with or without OVA-peptide257-264 (SIINFEKL; 1 nM) and IL-2 (500 IU/ml) (n = 3 mice, experiments performed in triplicates). (C, D) Time course of T cell activation marker expression (mean fluorescence intensity (MFI) for CD69 and CD25; (C) and estimated single cell glutamate secretion rates (∆Glu/∆t/cell; (D ) during repetitive stimulation of splenocytes from OT-I mice by incubation for time intervals of 72 hours with OVA-peptide257-264 (SIINFEKL; 1 nM) and IL-2 (500 IU/ml) (n = 3 mice, experiments performed in triplicates).

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