Figure 4

Caspase-3 activation in neurons exposed to glutamate. (A, B) Representative double staining for activated caspase-3 (red) in cultured neurons (NeuN, green; DAPI, blue) before (A ) and after exposure to 0 μM (B ) or 100 μM (C ) L-glutamate for six hours. (D ) Fraction of activated caspase-3⁺ neurons under distinct experimental conditions (n = 5 respectively).