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Figure 6 | Journal of Neuroinflammation

Figure 6

From: Excitotoxic neuronal cell death during an oligodendrocyte-directed CD8+ T cell attack in the CNS gray matter

Figure 6

Antigen-dependent glutamate release from CD8 + T cells causes collateral excitotoxic neuronal cell death during an oligodendrocyte-directed CD8 + T cell attack in the CNS gray matter. (A-F) Activated OT-I T cells were incubated for six hours [5] in acute brain slices from ODC-OVA mice in the absence (A, OT-I) or presence (B, OT-I NBQX MK801) of MK801 (10 μM) and NBQX (30 μM), and collateral neuronal cell death (white arrows) in the cortical gray matter was tracked via staining for NeuN (green), DAPI (blue) and activated caspase-3 (red). As controls, acute brain slices from ODC-OVA mice were left untreated (C, control), or incubated either with L-glutamate (D, Glu, 300 μM) or with MK801 and NBQX (E, NBQX MK801, 10 μM and 30 μM) for six hours. (F ) Fractions of activated caspase-3+ NeuN+ neurons detected in the cortical gray matter of acute brain slices from ODC-OVA mice under the distinct experimental conditions (n = 3 mice, each providing three brain slices for all experimental groups).

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