Figure 11
(A) Splenic apoptosis was not affected by global HI as indicated by unchanged numbers (cells/mm 2 ) of activated caspase-3 positive cells in the spleen assessed seven days after umbilical cord occlusion (UCO); (B) Sham, spleen; activated caspase-3; (C) HI, spleen; activated caspase-3; (D) Global HI did not change areal fraction (%) of MPO immunoreactivity in the spleen seven days after UCO; (E) Sham, spleen; MPO; (F) HI, spleen; MPO; (G) Global HI did not change areal fraction (%) of CD3 immunoreactivity in the spleen seven days after UCO; (H) Sham, spleen; CD3; (I) HI, spleen; CD3. Scale bar activated caspase-3 images = 50 μm. Scale bar CD3 and MPO images = 200 μm, scale bar inserts = 50 μm. (A, D, G) Means ± 95% CI are depicted. *P ≤0.05, # P ≤0.01, ‡ P ≤0.001, NS, non-significant. HI = hypoxia-ischemia