Figure 5

Global HI induced significant proliferation and activation of microglia in the cornu ammonis (CA)1-2, CA3 and dentate gyrus (DG), sub-regions of the hippocampus. (A) Global HI significantly increased areal fraction (%) of IBA-1 immunoreactivity (IR) in CA1-2; (B) Sham, CA1-2; resting microglia; (C) HI, CA1-2; profound microglial proliferation and activation; (D) Global HI significantly increased areal fraction (%) of IBA-1 IR in CA3; (E) Sham, CA3; resting microglia; (F) HI, CA3; profound proliferation and activation of microglia; (G) Global HI significantly increased areal fraction (%) of IBA-1 IR in DG; (H) Sham DG; resting microglia; (I) HI, DG; proliferation and activation of microglia. Scale bar images = 200 μm, scale bar inserts = 25 μm. (A, D, G) Geometric means ± 95% CI are depicted. *P ≤0.05, # P ≤0.01, ‡ P ≤0.001, NS, non-significant. HI = hypoxia-ischemia.