Effects of genetic deletion and blockade of FP receptor on the glial fibrillary acidic protein (GFAP) expression after controlled cortical injury (CCI). (A and B) Representative photographs of GFAP immunohistochemistry on hippocampal brain sections from sham- and CCI-injured WT (A) and FP-/- (B) mice 48 hours after injury. Rectangular selections in the inserts denote magnified regions. (C) Bar graph represents quantitative analysis of GFAP immunoreactivity in cortical penumbra, hippocampus, and thalamus in saline- and AL-8810- (10 mg/kg) treated WT and FP-/- mice that underwent CCI or sham 48 hours and 10 days following injury. All data are normalized to the corresponding value of the contralateral side. Data are presented as mean ± SEM, ***P < 0.001 versus saline-treated WT sham group, #P < 0.05, ##P < 0.01, versus saline-treated WT CCI group, one-way ANOVA followed by Tukey's multiple comparison test (n = 3 to 7).