Figure 2
From: Involvement of NT3 and P75NTRin photoreceptor degeneration following selective Müller cell ablation
Upregulation of P75NTRin transgenic retinas after Müller cell ablation. (A-I) Double-label immunostaining for GS (A, D and G) and P75NTR(B, E and H) in control (Ctl, A-C) and transgenic (TG, D-I) mice 7d and 14d after tamoxifen treatment. The retina of control mice showed weak immunoreactivity of P75NTR(A-C). TG mice showed upregulation of P75NTR predominantly by surviving Müller cells 7d (D-F) and 14d (G-I) after TMX-induced Müller cell ablation. Small arrows in (D and G) point to regions of Müller cell loss, and large arrows in (G) indicate defects in the outer limiting membrane following Müller cell ablation. GCL = ganglion cell layer, INL = inner nuclear layer, ONL = outer nuclear layer. (J and K) Western blots showed significant upregulation of GFAP concomitantly with overexpression of P75NTR 7d and 14d after Müller cell ablation in TG mice. †P < 0.05 and *P < 0.01, TG vs. control, N = 4–8 in each group. Scale bars: A-I, 50 μm.