Skip to main content
Figure 3 | Journal of Neuroinflammation

Figure 3

From: Involvement of NT3 and P75NTRin photoreceptor degeneration following selective Müller cell ablation

Figure 3

Differential expression of mature NT3, pro-NT3, rod and cone phototransduction proteins 7d after Müller cell ablation. (A, F) Western blots using antibodies to detect mature NT3, pro-NT3, rhodopsin, guanine nucleotide-binding protein subunit alpha-1 (GNAT1) and Gα protein transducin (Gαt). GNAT1 and Gαt are proteins essential for rod and cone phototransduction [2729]. (B-D) Quantitative analysis of protein densitometry showed significant reduction in mature NT3 (B) and upregulation of pro-NT3 (C), which resulted in a decreased ratio of NT3:pro-NT3 (D) 7d after Müller cell ablation. (E, G, H) The reduced expression of rhodopsin (E), GNAT1 (G) and Gαt (H) in transgenic (TG) mice indicates that selective Müller cell ablation causes damage to both rod and cone photoreceptors. *P < 0.05 and P < 0.01, TG vs. control (Ctl), n = 8/group in (B, D and E) and n = 11 − 13/group in (C, G and H), respectively.

Back to article page