Figure 7From: Involvement of NT3 and P75NTRin photoreceptor degeneration following selective Müller cell ablationInhibition of microglial activation after intravitreal injection of recombinant mature NT3 and blockage of P75NTR. Transgenic mice received twice intravitreal injections of NT3 (0.4 μg), P75NTR rabbit polyclonal a polyclonal Ab to P75NTR (1:1 dilution), a combination of both or balanced salt solution (BSS) 3d and 6d after tamoxifen (TMX)-induced Muller cell ablation. Eyes were enucleated 10d after TMX treatment for retinal flat-mount staining using an Ab against ionized calcium binding adaptor molecule 1 (IBA-1) for microglia (A, D, G and J, green) and peanut-agglutinin (PNA)-conjugated with Alexa Fluor-594 for cone photoreceptor outer segments (B, E, H and K, red). (C, F, I and L) Merged images. (M) Quantitative analysis of IBA-1 stained retinal whole mounts shows intravitreal injection of NT3 and P75NTR blockage, either alone or in combination, protected photoreceptors concurrently with inhibition of microglial activation. *P < 0.05 and †P < 0.01, all vs. BSS injected group, n = 9 − 11/group. Scale bars in A-L: 100 μm.Back to article page