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Figure 7 | Journal of Neuroinflammation

Figure 7

From: Involvement of NT3 and P75NTRin photoreceptor degeneration following selective Müller cell ablation

Figure 7

Inhibition of microglial activation after intravitreal injection of recombinant mature NT3 and blockage of P75NTR. Transgenic mice received twice intravitreal injections of NT3 (0.4 μg), P75NTR rabbit polyclonal a polyclonal Ab to P75NTR (1:1 dilution), a combination of both or balanced salt solution (BSS) 3d and 6d after tamoxifen (TMX)-induced Muller cell ablation. Eyes were enucleated 10d after TMX treatment for retinal flat-mount staining using an Ab against ionized calcium binding adaptor molecule 1 (IBA-1) for microglia (A, D, G and J, green) and peanut-agglutinin (PNA)-conjugated with Alexa Fluor-594 for cone photoreceptor outer segments (B, E, H and K, red). (C, F, I and L) Merged images. (M) Quantitative analysis of IBA-1 stained retinal whole mounts shows intravitreal injection of NT3 and P75NTR blockage, either alone or in combination, protected photoreceptors concurrently with inhibition of microglial activation. *P < 0.05 and P < 0.01, all vs. BSS injected group, n = 9 − 11/group. Scale bars in A-L: 100 μm.

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