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Figure 1 | Journal of Neuroinflammation

Figure 1

From: Optical tomographic imaging of near infrared imaging agents quantifies disease severity and immunomodulation of experimental autoimmune encephalomyelitis in vivo

Figure 1

Experimental autoimmune encephalomyelitis induction and imaging

Experimental autoimmune encephalomyelitis induction and imaging. SJL/J mice were immunized with PLP139-151 in CFA as described in the Materials and Methods section. Mice were monitored for clinical signs of disease and enrolled for imaging agent injection upon achieving a score of 2 or 3, and asymptomatic mice were excluded from imaging. Control mice (immunized with CFA only) were injected with imaging agents on day 14. All mice were imaged 24 h after imaging agent injection. For treatment studies, all mice (including asymptomatic mice from the positive control group) were injected on day 13 for imaging on day 14. Two separate FMT tomographic scans (head and spine) were performed, and excised brains and spinal cords were imaged by FMT epifluorescence to confirm in vivo results.

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