Figure 3
Quantifying BBB disruption. PLP139-151-immunized mice were injected intravenously with AS750 upon achieving a clinical score of 2 or 3 and imaged by FMT 4000 using tomographic scanning capabilities. CFA control mice were injected 14 days following immunization. (A) NIR tomographic fluorescent signal in 2–3-mm slices through the spinal cord and brain regions of representative control and diseased mice. (B) NIR tomographic quantification of the fluorescent signal (mean pmol ± SE) in the brains and spinal cord of all experimental mice. (C) Brains and spinal cord tissues of the same representative mice shown in A were imaged by epifluorescence on the FMT (left panel). Epifluorescence quantification (right panel) for tissues from all mice. Study is representative of three separate experiments, #p < 0.05, *p < 0.01, **p < 0.005).