Figure 4

Acute downregulation of the proinflammatory response 2 h after birth in all experimental groups. Increased IL-10 and IL-6 mRNA levels in non-preconditioned PA animals, but baseline IL-10 and IL-6 mRNA levels in preconditioned animals. Postnatal relative mRNA levels of IL-1β (A), TNF-α (B), IL-6 (C) and IL-10 (D) in control, FA, PA and FA-PA animals. mRNA levels are relative to the geomean of measured housekeeping genes and normalized to control levels. Relative protein expression of pSTAT3/STAT3 normalized to GAPDH and to respective control levels (E). # 0.08<p < 0.05; *p<0.05 and **p < 0.01 indicate significant differences between groups. Data shown as mean + SEM.