Figure 3

Novel environment stress (stress) induced nuclear NF-IL6-IR that co-localizes with CD163-positive perivascular macrophages in the rat pituitary. NF-IL6-IR (red) was co-localized with specific cell marker proteins (green) after novel environment-stress (90 min) or in unstimulated control animals (60 min). (A-H) Stress-induced nuclear NF-IL6-IR was not co-localized in corticotrope cells (ACTH; A, B), astrocytes (GFAP; C, D), endothelial cells (VWF; E, F) but in perivascular macrophages (CD163; H) of the anterior (AL, c) and posterior pituitary lobe (PL, a). Insets (a-c) show high magnifications of the PL, IL, and AL, respectively. Open arrows represent either NF-IL6-IR-negative cells of identified phenotype or some NF-IL6-IR cells of unidentified phenotype (green). White arrow tips show nuclear NF-IL6-IR in perivascular macrophages. Cell nuclei were labeled with DAPI (blue). Brightness, contrast, and color balance were adjusted for better representation of the actual data. The scale bar in A 100 μm (applies to A-H) and 10 μm for all insets (a-c).