Figure 6
Co-localization of nuclear NF-IL6-IR with NFκB- or STAT3-IR 2 or 4 h after LPS-stimulation. (A, B) The overviews depict the neuroanatomical structure and distribution pattern of IR in the anterior lobe (AL), the intermediate lobe (IL), and the posterior lobe (PL) of the rat pituitary. Dashed squares indicate the localization of high magnification (a, b). LPS (100 μg/kg i.p.) induced nuclear (DAPI, blue) NF-IL6-IR (red), which shows some co-localization with both STAT3- and NFκB-IR (both green) as indicated by open arrows in single channels (a 1-2 -b 1-2 ) and the merged overlay (a 3 -b 3 ) 2 (NFκB) or 4 h (STAT3) after stimulation, respectively. Moreover, nuclear NF-IL6-IR can also be seen without co-localization (white arrows) and some single nuclear NFκB-IR occurred with only very weak co-localization with NF-IL6-IR (white arrow tips). Brightness, contrast, and color balance were adjusted for better representation of the actual data. Scale bar in A represents 100 μm (applies to A, B), 10 μm in a1 and applies to a 1-3 and b 1-3 .