Figure 2

Immunohistochemical analysis of the effect of the subacute LPS treatment on CD11b-positive microglia. PBS (2 μL/injection, bilaterally) or LPS (5 μg/2 μL/injection, bilaterally) was injected into the SNR daily for 5 consecutive days. (A) Representative photomicrographs are coronal sections of the SNC 6 hr after the final PBS (upper rows) or LPS treatment (lower rows). Double immunofluorescence staining was performed with a CD11b antibody (green) and DAPI (blue). Scale bars: 50 μm (B) Number of CD11b immunopositive cells in the SNC. The number of microglial cells was counted between 200 μm and 300 μm from the injection site. The results are expressed as the mean ± SEM for four mice in each group. DAPI, 4',6-diamidino-2-phenylindole; IL, interleukin; LPS, lipopolysaccharide; PBS, phosphate-buffered saline; SEM, standard error of the mean; SNC, substantia nigra pars compact; SNR, substantia nigra pars reticulata.