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Figure 3 | Journal of Neuroinflammation

Figure 3

From: Activation of microglia induces symptoms of Parkinson’s disease in wild-type, but not in IL-1 knockout mice

Figure 3

Immunohistochemical analysis of the effect of subacute LPS treatment on oxidative stress markers. PBS (2 μL/injection, bilaterally) or LPS (5 μg/2 μL/injection, bilaterally) was injected into the SNR daily for 5 consecutive days. (A) Representative photomicrographs are coronal sections of the SNC region 6 hr after the final PBS (upper row) or LPS treatment (lower row). Double immunofluorescence staining was performed with 4HNE (red, upper row) or NT (red, lower row) antibody and DAPI (blue). Scale bars: 20 μm (B) Numbers of 4HNE and NT immunopositive cells in the SNC. The results are expressed as the mean ± SEM for four mice in each group. 4HNE, 4-hydroxynonenal; DAPI, 4',6-diamidino-2-phenylindole; LPS, lipopolysaccharide; PBS, phosphate-buffered saline; SEM, standard error of the mean; SNC, substantia nigra pars compact; SNR, substantia nigra pars reticulata.

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