Figure 3

Effect of YKS on the expression of activated microglia in the DG of Gunn rats. YKS suppressed microglial activation in the DG of Gunn rats. A confocal Z-stack image depicting Iba-1 (red), CD11b (green), and a merged image (yellow) of cells in the DG of control Gunn rats (A–D) and YKS-treated Gunn rats (E–H). Microglial cells in YKS-treated Gunn rats exhibited a low level of CD11b immunoreactivity (IR) compared with those in control Gunn rats (C, G). Scale bar, 100 μm (A, E), 50 μm (B–D, F–H). (I) Mean percentage of black pixels indicating CD11b immunoreactivity in the DG. Data are presented as the mean ± SEM (n = 4, respectively). *P <0.05, ANOVA followed by Bonferroni post hoc test.