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Table 2 Summary of quantitative neuropathological analysis of microglia

From: Clinically relevant intronic splicing enhancer mutation in myelin proteolipid protein leads to progressive microglia and astrocyte activation in white and gray matter regions of the brain

Brain region

C57BL/6 J

PLP-ISEdel

Two-way ANOVA

2mo

4mo

2mo

4mo

Geno

Age

Inter

IBA1 IHC

Cerebellum gm

100 ± 17

293 ± 68

82 ± 24

293 ± 68

ns

**

ns

Cerebellum wm

100 ± 35

424 ± 181

235 ± 35

1,058 ± 297

*

**

ns

Medulla

100 ± 21

324 ± 127

272 ± 41

940 ± 216

**

**

ns

Pons

100 ± 23

237 ± 116

274 ± 52

512 ± 55

**

*

ns

Midbrain

100 ± 35

243 ± 71

261 ± 26

540 ± 131

**

*

ns

Thalamus

100 ± 30

161 ± 55

193 ± 14

256 ± 75

ns

ns

ns

Striatum

100 ± 24

323 ± 103

87 ± 7

335 ± 74

ns

**

ns

Corpus callosum

100 ± 18

195 ± 41

152 ± 44

294 ± 84

ns

*

ns

Cortex

100 ± 14

143 ± 17

81 ± 16

136 ± 22

ns

*

ns

Hippocampus

100 ± 34

200 ± 51

76 ± 4

141 ± 38

ns

*

ns

CD68 IHC

Cerebellum gm

100 ± 47

500 ± 194

375 ± 88

1,539 ± 398

**

**

ns

Cerebellum wm

100 ± 31

346 ± 67

1,464 ± 127

11,607 ± 214

****

****

***

Medulla

100 ± 11

323 ± 35

1,571 ± 166

7,888 ± 1294

****

****

***

Pons

100 ± 20

433 ± 50

1,161 ± 83

6,121 ± 1107

****

****

***

Midbrain

100 ± 21

260 ± 26

515 ± 76

2,960 ± 379

****

****

****

Thalamus

100 ± 10

251 ± 19

498 ± 78

2,075 ± 257

****

****

****

Striatum

100 ± 29

218 ± 17

157 ± 42

1,142 ± 213

***

****

**

Corpus callosum

100 ± 15

208 ± 22

690 ± 96

2,715 ± 285

****

****

****

Cortex

100 ± 25

427 ± 115

308 ± 62

1,301 ± 285

***

****

**

Hippocampus

100 ± 45

320 ± 24

104 ± 16

513 ± 50

**

****

**

MHCII IHC

Cerebellum gm

100 ± 46

336 ± 63

797 ± 320

632 ± 386

ns

ns

ns

Cerebellum wm

100 ± 75

296 ± 156

493 ± 275

891 ± 223

ns

*

ns

Medulla

100 ± 40

351 ± 57

556 ± 281

1,551 ± 280

*

*

ns

Pons

100 ± 53

437 ± 100

1,587 ± 211

963 ± 291

***

ns

*

Midbrain

100 ± 33

99 ± 22

970 ± 250

1,139 ± 185

****

ns

ns

Thalamus

100 ± 36

109 ± 25

569 ± 200

841 ± 251

**

ns

ns

Striatum

100 ± 25

61 ± 14

326 ± 150

790 ± 318

*

ns

ns

Corpus callosum

100 ± 36

65 ± 19

463 ± 98

2,505 ± 1148

ns

ns

ns

Cortex

100 ± 38

197 ± 54

398 ± 126

474 ± 87

**

ns

ns

Hippocampus

100 ± 47

738 ± 232

1,482 ± 692

1,677 ± 839

ns

ns

ns

  1. 2-month-old WT mice used as 100%. Mean ± SEM for each brain region are indicated. (n = 5 to 6 per group). Abbreviations: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; geno = genotype; gm = gray matter; inter = interaction of age and genotype; mo = months-old; ns = not significant; wm = white matter.