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Table 2 Summary of quantitative neuropathological analysis of microglia

From: Clinically relevant intronic splicing enhancer mutation in myelin proteolipid protein leads to progressive microglia and astrocyte activation in white and gray matter regions of the brain

Brain region C57BL/6 J PLP-ISEdel Two-way ANOVA
2mo 4mo 2mo 4mo Geno Age Inter
IBA1 IHC
Cerebellum gm 100 ± 17 293 ± 68 82 ± 24 293 ± 68 ns ** ns
Cerebellum wm 100 ± 35 424 ± 181 235 ± 35 1,058 ± 297 * ** ns
Medulla 100 ± 21 324 ± 127 272 ± 41 940 ± 216 ** ** ns
Pons 100 ± 23 237 ± 116 274 ± 52 512 ± 55 ** * ns
Midbrain 100 ± 35 243 ± 71 261 ± 26 540 ± 131 ** * ns
Thalamus 100 ± 30 161 ± 55 193 ± 14 256 ± 75 ns ns ns
Striatum 100 ± 24 323 ± 103 87 ± 7 335 ± 74 ns ** ns
Corpus callosum 100 ± 18 195 ± 41 152 ± 44 294 ± 84 ns * ns
Cortex 100 ± 14 143 ± 17 81 ± 16 136 ± 22 ns * ns
Hippocampus 100 ± 34 200 ± 51 76 ± 4 141 ± 38 ns * ns
CD68 IHC
Cerebellum gm 100 ± 47 500 ± 194 375 ± 88 1,539 ± 398 ** ** ns
Cerebellum wm 100 ± 31 346 ± 67 1,464 ± 127 11,607 ± 214 **** **** ***
Medulla 100 ± 11 323 ± 35 1,571 ± 166 7,888 ± 1294 **** **** ***
Pons 100 ± 20 433 ± 50 1,161 ± 83 6,121 ± 1107 **** **** ***
Midbrain 100 ± 21 260 ± 26 515 ± 76 2,960 ± 379 **** **** ****
Thalamus 100 ± 10 251 ± 19 498 ± 78 2,075 ± 257 **** **** ****
Striatum 100 ± 29 218 ± 17 157 ± 42 1,142 ± 213 *** **** **
Corpus callosum 100 ± 15 208 ± 22 690 ± 96 2,715 ± 285 **** **** ****
Cortex 100 ± 25 427 ± 115 308 ± 62 1,301 ± 285 *** **** **
Hippocampus 100 ± 45 320 ± 24 104 ± 16 513 ± 50 ** **** **
MHCII IHC
Cerebellum gm 100 ± 46 336 ± 63 797 ± 320 632 ± 386 ns ns ns
Cerebellum wm 100 ± 75 296 ± 156 493 ± 275 891 ± 223 ns * ns
Medulla 100 ± 40 351 ± 57 556 ± 281 1,551 ± 280 * * ns
Pons 100 ± 53 437 ± 100 1,587 ± 211 963 ± 291 *** ns *
Midbrain 100 ± 33 99 ± 22 970 ± 250 1,139 ± 185 **** ns ns
Thalamus 100 ± 36 109 ± 25 569 ± 200 841 ± 251 ** ns ns
Striatum 100 ± 25 61 ± 14 326 ± 150 790 ± 318 * ns ns
Corpus callosum 100 ± 36 65 ± 19 463 ± 98 2,505 ± 1148 ns ns ns
Cortex 100 ± 38 197 ± 54 398 ± 126 474 ± 87 ** ns ns
Hippocampus 100 ± 47 738 ± 232 1,482 ± 692 1,677 ± 839 ns ns ns
  1. 2-month-old WT mice used as 100%. Mean ± SEM for each brain region are indicated. (n = 5 to 6 per group). Abbreviations: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; geno = genotype; gm = gray matter; inter = interaction of age and genotype; mo = months-old; ns = not significant; wm = white matter.