Figure 2

Unilateral tactile allodynia observed following L5 SNL in C57BL/6 mice reduced in TLR signaling deficient mice. L5 SNL was performed on (A) C57BL/6, (B) Tlr2 ^-/-, (C) Tlr3 ^-/-, (D) Tlr4 ^-/-, and (E) Tlr5 ^-/- mice. Mice were allowed to recover and were tested at days 7, 9, 12 and 14 post-SNL. The solid black line and dashed line represent the C57BL/6 ipsilateral and contralateral thresholds, respectively, on B-E. (A) C57BL/6 mice show a robust tactile allodynia in the ipsilateral paw beginning 7-days post-surgery. The (B) Tlr2 ^-/-, (C) Tlr3 ^-/-, (D) Tlr4 ^-/-, and (E) Tlr5 ^-/- all produced a reduction in the ipsilateral paw tactile threshold following L5 SNL, but none completely reversed nerve injury-induced allodynia. Data are expressed as mean ± SEM (n = 5–8 mice/group) and analyzed via 2-way ANOVA, followed by Bonferroni post hoc test to compare each time point to the respective WT C57BL/6 group, ipsilateral or contralateral (*P <0.05 or **P <0.01).