Figure 6

MyD88 signaling deficient mice have less ATF3+ nuclei following L5 SNL. At day 14 following L5 SNL, the ipsilateral and contralateral L5 DRGs were harvested and incubated with an antibody against ATF3. (A) Myd88 ^-/- DRGs display less ATF3 immunoreactivity in their L5 ipsilateral DRGs when compared to C57BL/6. No significant difference was found among the other groups. Data expressed as mean ± SEM (n = 4–8 mice/group) and analyzed via 2-way ANOVA followed by Bonferroni post hoc test. The Myd88 ^-/- ipsilateral group is statistically different from the C57BL/6 ipsilateral group (**P <0.01). Representative stained images from the DRGs of C57BL/6 (B-D), Myd88 ^-/- (E-G), and Trif ^lps2 (H-J) mice are presented. Examples of ATF3+ nuclei are marked with a white arrow in D, G, and J.