Figure 7

IRF3 and IFNR signaling is required for regulation of contralateral tactile allodynia following L5 SNL. L5 SNL was performed on (A) Irf3 ^-/-, and (B) Irf7 ^-/-, (C) Tnf ^-/-, and (D) Ifnar ^-/- mice. Mice were allowed to recover and were tested at days 7, 9, 12, and 14 post-SNL. The solid black line and the dashed line represent the C57BL/6 ipsilateral and contralateral thresholds, respectively. The Irf3 ^-/- (A) and Ifnar ^-/- (D) mice displayed a tactile allodynia in both the ipsilateral and contralateral paws. Conversely, the Irf7 ^-/- (B) and Tnf ^-/- (C) mice only produced a drop in the ipsilateral paw threshold. There was no statistical significance between the C57BL/6 mice and these mice. Data expressed as mean ± SEM (n = 5–8 mice/group) and analyzed via 2-way ANOVA, followed by Bonferroni post hoc test to compare each time point to the respective C57BL/6 group, ipsilateral or contralateral (*P <0.05 or **P <0.01 for ipsilateral group; ##P <0.01 for contralateral group).